FastQCFastQC Report
Wed 31 May 2023
EGAF00007951233

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007951233
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences444103
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT77631.7480179147630166No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA59891.3485610320128438No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC30270.6815986381537616No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA26400.5944566913531321No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA22150.49875817096484376No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA20520.4620549737335708No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA19680.443140442645062No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19630.4420145776993175No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT18980.42737833340463816No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG16810.37851579475932384No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC16640.3746878539437923No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG15750.354647457909539No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT15380.3463160573110292No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC12680.28551935024082253No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC10910.24566373116146478No Hit
GGCTACCAGGAACCACCGGCACCACAAGCCC10260.2310274868667854No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC10120.2278750650187006No Hit
TCCCGTGAGGCACTGGAACTGCGCGACGGTG9810.2208947023550843No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG9780.22021918338763755No Hit
GGTTACCAGGAACCGCCGGCACCACAGGCGC9340.21031157186508537No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG8560.1927480787114701No Hit
AACGATGGCTCCGTAGAATGGCAGTACCAGG7610.1713566447423233No Hit
GTGGCACAGTCCCAGTACTTCGACCTGCCGC7420.16707835794849393No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC7420.16707835794849393No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT7170.16144903321977108No Hit
CCGACTGGTACGATCCCGGATATGCGTGGCC6990.15739591941509065No Hit
CCGCTGCCGCCGGCAACGCTGACCGTTCCGC6540.14726313490338955No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA6440.1450114050119004No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA6420.14456105903360256No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG5680.12789825783658296No Hit
CGTGGCCAGAGCCGTGGCCGTGGCCGTGGTC5660.12744791185828513No Hit
CCGAACACCTCTAGCCCGTCTATGCCGGAAC5370.12091789517296664No Hit
CGTGTTCGTGGTCACTACCGTGCTCCGTGGG4850.10920889973722311No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC4820.1085333807697764No Hit
ACTCCGTCTGACGCAGGCGAAACGGTCGCGG4710.10605647788913831No Hit
GGTCGTCAGGGCAGCGACGTAGAACTGGAAT4640.10448026696509594No Hit
GCTTCCGAACCGGAGGACAAATCTCCGCGCG4600.10357957500850028No Hit
GCAAGCGAACCGGAGGACAAATCTCCGCGCG4570.10290405604105354No Hit
CGCGGCCGTGGTAAAGGTAAGTCTCGTGATA4510.10155301810616006No Hit
GGTGAACTGGCTGCATCTGCGGCATCCCTGA4500.10132784511701115No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)