FastQCFastQC Report
Wed 31 May 2023
EGAF00007951253

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007951253
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences503486
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCGCCACACCCAGAAAGCACCGAAACGTA41280.8198837703530982No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC26760.5314944208975025No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG21610.4292075648578113No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT20260.402394505507601No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA19640.3900803597319489No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA17700.3515490003694244No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT14600.2899782714911636No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA14550.28898519521893357No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA14250.2830267375855535No Hit
GGTCGTCAGGGCTCCGATGTCGAACTGGAAT13770.27349320537214544No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT13740.2728973596088074No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG13720.2725001290999154No Hit
GTTGCGACCCCACACGCCTCCGCGCGCGCTC11960.23754384431741898No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG11940.23714661380852695No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT11430.22701723583178082No Hit
GGTCGTCAGGGCAGCGACGTAGAACTGGAAT11100.22046293243506276No Hit
ATTGCTGCCGTTGGCTCTCCAGTGAAGTCTA10760.21371001378389867No Hit
GCAGAAGCGCAGTCCACTCCGGAGCGTCCTG10540.20934047818608661No Hit
GAACTGGTGACCACCGAACGCAAAACTCCGC9450.18769141545147233No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9400.1866983391792423No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA8810.17498003916692817No Hit
GCTTGGGCGCTGAAAAACCCGCACCTGGCAT8610.17100773407800812No Hit
AGCCCGGCTAAATCTGCGCCGCCGTCTCCGG8490.1686243510246561No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG8270.16425481542684403No Hit
TCCCGCGTTCCGGCTAAAAAACCGCGTAAAC8110.161076971355708No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT7850.15591297474011193No Hit
TTCGAACAGCCGACCCTGACCTTCGGCGCGG7740.1537282069412059No Hit
GACAAAGCTGAAGCACAGTCCACTCCGGAAC7610.15114620863340789No Hit
GGTCGTCAGGGCTCCGACGTCGAGCTGGAAA7430.14757113405337985No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG7340.1457835967633658No Hit
TCTCGCGTGCCGGCGAAGAAACCGCGTAAAC7140.14181129167444578No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG7110.14121544591110777No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG6910.1372431408221877No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG6500.12909991538990162No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG6250.12413453402875155No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC6180.12274422724762953No Hit
GCACCGAAGCCTTCCAAACAGAGCAAGAAGA5900.11718300012314145No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC5570.11062869672642336No Hit
GACCCAGCTATCGTTCAGCCGAAAGCAGCAA5530.10983423570863936No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT5430.10784808316417933No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG5370.10665639163750333No Hit
CGTGATCTGGAAGCGGTACGCCAGGCCAAAA5320.1056633153652733No Hit
GATGGTTACATCTTCAACCCGAAAGACATCG5230.1038757780752593No Hit
CACGAAGCTCCGAACATGACCCAGACCGGTA5210.10347854756636728No Hit
ATGGAGCCAGTTCAGCAGCGCCCGGTTATGT5070.10069793400412325No Hit
TCTACCGAAAGCGTTGACAAAAACTTTGTTG5060.10049931874967724No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)