FastQCFastQC Report
Sat 22 Apr 2023
EGAF00007951264

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007951264
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences536535
Sequences flagged as poor quality0
Sequence length44
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA33380.6221402145246815No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA30580.5699534979078719No Hit
CGGCGGCGGTTCCTGATAACCCTGGTACGGGGCTTGCGGAGCCG24100.4491785251661122No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT23050.4296085064348086No Hit
TGGCGGCGGTTCCTGGTAGCCCTGATACGGTGCCTGCGGCGCCG18210.33940003914003747No Hit
GTGCTGGGAACGTGGGGTCCACGGGCCTGCATAACCCGGGTAGG15090.2812491263384495No Hit
GGCGCCCGCGGTTCAACCAATGGTAACACAAGAACTGAGCCTTC14550.27118454527663616No Hit
AGAACTGAGCCTTCACTGGTTCAGCTCCCTCGGTCGAGTACGCT13010.24248185113739085No Hit
CGGCGGAACCGGGACAGTAGCCTGCGCGGTGGTCACCGGTGGGG12680.2363312738218383No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT11800.21992973431369808No Hit
CAGCGTCGGAATGGAGCGACGGGTCTTGATACCGTTGCCGCTTT11560.21545658717511437No Hit
CGAGATGTTTCCATCCAGAGGTCTCCGCAGCGGAAAAAATTGGC11080.20651029289794703No Hit
GCTAGTTATAGCGGGGCCGAGGTTACTAACCAAAGTGTCGGCAC9660.18004417232799352No Hit
ACCAGTAGCAACTACCGCCAGATCGCGGTTCGGGTACGGTGCAA9630.17948502893567053No Hit
GTTGACCCATTCGCCGTTAGCGTTAACACCATAGCCGTCAACGG9340.17407997614321527No Hit
GAAGATGTAATCCCAGGACTCATCCAGGTCAGCCGGATCGATAG9130.17016597239695452No Hit
GTGCTGGCTACGCGGGGTCCACGGACCAGCATAGCCCGGGTAGG9010.1679293988276627No Hit
ATACGGATAAGAACTACGGGCAGTGGCTGTAACCCTCTTAACCG8180.15245976497339409No Hit
CTGTGCGCCCTACGCGGAGGTGGCCTATTCATCGGTGCAGGATT7860.14649556878861583No Hit
CAGAGCGAAAGTAATGTTTTTGCCATCACGCTTAACCTTCAGGT7790.1451909008731956No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC7670.14295432730390376No Hit
GTGGGAGTTACGGCATACCGGTGCCGCGTTAGATGGACCCGGGG7480.13941308581919165No Hit
GATTGCCGGAGATGGAGTTTCCGGACGCGCCGGAGATTCCCAGG7340.13680374998835118No Hit
AGAGGACGGACGGCTACCCTGTTTGCCGTTCCAGTAGGCCTGTG7240.134939938680608No Hit
GATAACGAAGCTAGCGGAGGTCGGGGTAATGACCACAGAGGACG7140.13307612737286478No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT7020.13083955380357293No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT6820.1271119311880865No Hit
AACCCTCTTAACCGCTGTATCGGCGAGAAGCTCTTCTGAGGGGC6780.12636640666498922No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA6670.12431621422647171No Hit
CGGCGCACCGTGGTTGATCGGCTGGGTCAGCGGCAGGTCGAAGT6370.1187247803032421No Hit
ACGCATTGCGCCGTTAGACGGGTTCAGATAGTACCAAGTGGAAC6290.11723373125704754No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA6210.11574268221085297No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT5760.10735553132600856No Hit
TTCCATCGGCTGCTGCGGGCTTACCTGGGTCAGCTGCGGTGGCT5630.10493257662594238No Hit
CAGATTTTCTTCGTGTGCGTCCACGTTGTTATGGTTGTAGGAGT5570.10381428984129647No Hit
CGGAGCCTGCGGTGGCGGCGGCTCTTGATAACCCTGATACGGCG5420.10101857287968166No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)