FastQCFastQC Report
Sun 21 May 2023
EGAF00007951268

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007951268
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences534375
Sequences flagged as poor quality0
Sequence length44
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAACTGAGCCTTCACTGGTTCAGCTCCCTCGGTCGAGTACGCT42120.7882105263157895No Hit
GGCGCCCGCGGTTCAACCAATGGTAACACAAGAACTGAGCCTTC40340.7549005847953216No Hit
CGAGATGTTTCCATCCAGAGGTCTCCGCAGCGGAAAAAATTGGC40060.7496608187134504No Hit
ATACGGATAAGAACTACGGGCAGTGGCTGTAACCCTCTTAACCG28500.5333333333333333No Hit
AACCCTCTTAACCGCTGTATCGGCGAGAAGCTCTTCTGAGGGGC21580.40383625730994155No Hit
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA21470.4017777777777778No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT19440.3637894736842105No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA19290.3609824561403509No Hit
CGGCGGCGGTTCCTGATAACCCTGGTACGGGGCTTGCGGAGCCG11990.22437426900584795No Hit
TTACGCGTTGACAATACCGTAGGTCGTCAGCGAGATGTTTCCAT9010.16860818713450293No Hit
TGGCGGCGGTTCCTGGTAGCCCTGATACGGTGCCTGCGGCGCCG8770.16411695906432747No Hit
GTATTTCACGGAGCCCTGACCAGACGCAGTACCACCGCCGCGCG8060.15083040935672515No Hit
GTGCTGGGAACGTGGGGTCCACGGGCCTGCATAACCCGGGTAGG7940.14858479532163743No Hit
AGAAGCAACCGCGCTAACCGCGTCTTCCAGCTGCAGGGTCTGCT7240.13548538011695907No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG6630.12407017543859648No Hit
AGAGGACGGACGGCTACCCTGTTTGCCGTTCCAGTAGGCCTGTG6590.12332163742690058No Hit
GAAGATGTAATCCCAGGACTCATCCAGGTCAGCCGGATCGATAG6590.12332163742690058No Hit
GGTTGCCAGCGGACCAATGATAGTCGGCGGGGTTTTCGGACGGC6540.12238596491228071No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG6160.11527485380116959No Hit
GCTAGTTATAGCGGGGCCGAGGTTACTAACCAAAGTGTCGGCAC5990.112093567251462No Hit
GGCCTGTTCCTGGTTGGTAATGGACTGCGGAGTTTCGACTTCAA5940.11115789473684211No Hit
AAAGCCATCCTGTTTAGCTTTATTGATGATGTCCTTGATGGAGT5810.10872514619883042No Hit
GTGCTGGCTACGCGGGGTCCACGGACCAGCATAGCCCGGGTAGG5730.1072280701754386No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)