FastQCFastQC Report
Sun 21 May 2023
EGAF00007951275

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007951275
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences589715
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCGCCACACCCAGAAAGCACCGAAACGTA52640.892634577719746No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC35750.606225040909592No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT31200.5290691266120074No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT30380.5151641046946406No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA26100.4425866732235063No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT25840.4381777638350729No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG24830.42105084659538927No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA23010.39018848087635555No Hit
ACCTACCTGACGCAGGTACCGCCGGAACGTG20750.3518648838845883No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA19440.32965076350440464No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG18920.32083294472753787No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT16010.27148707426468716No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA15370.26063437423162034No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG14470.24537276481012013No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG14260.24181172261177009No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC14230.2413030022977201No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC12890.21858016160348645No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG12880.21841058816546977No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG12710.21552783971918638No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG12530.21247551783488633No Hit
GCCAACGACGTCTCCGGTCAGTCTGGCTTCA11710.19857049591751946No Hit
AAACAGCCAACCACCACCTCCCCGATCCACA11020.1868699286943693No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA10910.18500462087618597No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG10190.17279533333898578No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9580.16245135361996896No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA9580.16245135361996896No Hit
GATGGTTACATCTTCAACCCGAAAGACATCG9510.1612643395538523No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC9310.1578728707935189No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT9150.1551596957852522No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG8970.15210737390095214No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG8940.15159865358690214No Hit
CCTACCGATGGCCCGCCGCACCGTGGTGGCG8240.13972851292573532No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG8210.13921979261168532No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT8160.13837192542160195No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC8130.13786320510755196No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC7510.12734965195051848No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT7430.1259930644463851No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG7240.12277116912406841No Hit
CTGCGTGTCTGCGCTGCGCTGTCCATCCCGA7220.12243202224803507No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC7150.1212450081819184No Hit
CTGGACAAACTGACTCAGTCCCTGGACTCCG7000.11870140661166834No Hit
TATCACCAGAACCAGGGTGAAAACATTTCCA6670.11310548315711826No Hit
GCCGAAGCTGATTATTTCGAATACCACCAGG6450.10937486752075154No Hit
GATGGTGAACGTAACAACCAGGTTCTGGAAG6040.10242235656206812No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)