FastQCFastQC Report
Fri 21 Apr 2023
EGAF00007951283

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007951283
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences412421
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT75091.820712330361451No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG28650.6946784960028709No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC25880.6275141178552984No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG21680.5256764325773906No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21360.517917370841931No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG20270.49148806680552154No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG19460.47184794178763934No Hit
CAGGGCGCGCTGACCCTGTCCCTGCCGAAAC18660.4524502874489902No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC17180.4165646269224894No Hit
CAGGACTCTCTGCCGGACACTAAAGCCTCTG17100.41462486148862454No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG16400.39765191394230653No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC12510.30333081972062526No Hit
CAGGGTGCCCTGACTCTGAGCCTGCCGAAAC12500.30308834904139215No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA12480.30260340768292593No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG12400.300663642249061No Hit
GCTCTGACCCTGAGCCTGCCGAAACAGCAGG11700.28369069470274305No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG11630.28199339994811123No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT11350.2752042209295841No Hit
ATTGAGCAGAAACAGCTGCTGCAGGGTGATG10950.26550539376025956No Hit
GCGCTGACTCTGTCCCTGCCGAAACAGCAGG10830.26259574560946214No Hit
GCACTGACCCTGAGCCTGCCGAAACAGCAGG10750.26065598017559727No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG10070.24416797398774553No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT9420.22840737983759316No Hit
CAGAAACAGCTGCTGCAGGGTGACGTGGAAG8680.2104645495743427No Hit
CACAAAGCAAAACACATCAAGGCCTGGTGCC8640.20949466685741025No Hit
GAAAGCGTCGAACGTGCTATGGGTCGTGTTG8480.20561513598968045No Hit
ACCCAGAACCCAGTGGAAAACTACATCGACT8350.20246301715964996No Hit
ATGGGTCGCGTAGCAGACACTATCGCTCGTG8190.19858348629192016No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC7910.19179430727339297No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC7870.1908244245564605No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG7820.18961207116029494No Hit
CGTGACACCACTCACATTTCCCAGAGCGCAC7290.17676112516093992No Hit
GTTGCACGCGTCGCCGACACCCTGCCAACGG7260.17603371312324056No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT6830.1656074739162167No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC6770.16415264984081798No Hit
GAAGTATACATGAACGACCTGGGCACCGATA6690.16221288440695308No Hit
ATGGGTGCAGACATGGTAGAACTGGACATTC6140.14887699704913185No Hit
ACCCTGTCCCTGTCTCAGCTGTACTCCCACA6020.1459673488983345No Hit
CCGGAATCCTATTCCGGTGAACTGGAATGCA5600.1357835803705437No Hit
GGCCCGGCTCACTCCAAAGAAGTTCCGGCAC5500.13335887357821255No Hit
GAGAAAACGAAACAGACTCTGGCTTCTGTTT4760.11541604331496214No Hit
GGTCCAGCTCACTCTAAAGAAGTGCCGGCGC4720.11444616059802969No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA4680.11347627788109722No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC4560.11056662973029986No Hit
CACACCGCATACGCGAACTCTATCGATGGCA4530.10983921769260052No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC4400.10668709886257004No Hit
CCGGATCCGGCTGTTGCCCCGACCAGCGCGG4350.10547474546640447No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT4330.10498980410793826No Hit
GCTCCGACCAGCGCGGCGTCTCGCAAACCTG4300.10426239207023891No Hit
GAAGCCGTGAACCGTGCGGTGGCTCGTGTCG4150.1006253318817422No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)