FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007951295

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007951295
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences536872
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT75941.4144898597803572No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC47370.882333219091329No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA29800.5550671295951363No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG25270.4706894753311776No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT22960.4276624595806822No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG17940.33415786258177No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG17650.32875620259577704No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC17100.31851167503613526No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA14140.2633774903515177No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG14090.26244616966427753No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC12420.23134005871045613No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG12030.22407575734998286No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG10600.19743998569491422No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC10560.19669492914512213No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC10550.19650866500767408No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG10360.19296964639616146No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9560.17806851540031887No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT9550.17788225126287086No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC9460.17620587402583857No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG8860.16503002577895665No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG8510.1585107809682755No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC7890.1469624044464975No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC7810.14547229134691322No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC7530.14025689549836834No Hit
CCGGTAGGCGAAGCAGACTACTTCGAGTATC6790.12647334932721394No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG6770.12610082105231787No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG6700.12479697209018163No Hit
ATTGAGCAGAAACAGCTGCTGCAGGGTGATG6480.12069916106632493No Hit
AGCGGTGACTGTGGCGGCATCCTGCGCTGCC6440.1199541045165328No Hit
TCCTACACCAACGGCAAACTGACTATCACCC6390.11902278382929264No Hit
CCACGTCGCCCGCCGCCGGGCCGTCGTCCTT6350.11827772727950052No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA6310.1175326707297084No Hit
GATATTCGTGTTTACGGCATGAACGGCTGCA6020.11213101074371545No Hit
GCCGAAGCTGATTATTTCGAATACCACCAGG5960.11101342591902726No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG5720.10654308662027448No Hit
CAGGGCGCGCTGACCCTGTCCCTGCCGAAAC5510.1026315397338658No Hit
CCTGTAGGTGAAGCGGACTACTTCGAGTATC5390.10039637008448941No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)