FastQCFastQC Report
Wed 31 May 2023
EGAF00007951317

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007951317
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences504101
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCGCCACACCCAGAAAGCACCGAAACGTA77601.539374054009018No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT61821.2263415466345038No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC43420.8613353276426748No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA42830.8496313238815236No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG38560.7649260763220069No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA37310.740129458183975No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG25880.5133891819298118No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA25050.49692422748615855No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT23770.4715324905128139No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG20540.40745802924413954No Hit
CGTCGCCAGCGTGCAATCGACAAACGCCAGC20010.39694426315361403No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT17780.35270709639536524No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC17720.3515168587247397No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG15590.3092634214175334No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA12360.24518896014885908No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG12110.24022963652125268No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT11870.23546868583875055No Hit
AACCCGGAACACCTGAAGGAAGGCACTCAGA11760.23328658344260378No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC10500.20829159235946762No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC10170.20174528517102724No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT9310.1846852118920613No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8950.17754378586830813No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC7720.1531439136204848No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC7710.15294554067538052No Hit
TCCTACACCAACGGCAAACTGACTATCACCC7700.15274716773027627No Hit
ACTACCGACCCGGATGCAGCGGCTTCCGCGA7410.14699435232225289No Hit
GGTGTGCCGTCTGCCGGCCTGAAGGTTGGCG7310.14501062287121033No Hit
GCACGTCGTCAGGAAGCAGAACGCCAGGCCG7210.14302689342016778No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG7160.14203502869464651No Hit
CCACGTCGCCCGCCGCCGGGCCGTCGTCCTT6860.13608384034151888No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG6490.12874404137266143No Hit
GCCGAAGCTGATTATTTCGAATACCACCAGG6480.12854566842755716No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC6310.12517332836078485No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG5910.11723841055661463No Hit
TTCCCGGTGCTGGCGAACGAGGGCAAATCTG5630.11168396809369552No Hit
GCAGAAGCGCAGTCCACTCCGGAGCGTCCTG5570.11049373042306997No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA5540.10989861158775723No Hit
GCAGCTAACGTTGTACAGGTAGTTCAGAAAA5500.10910511980734018No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC5460.10831162802692318No Hit
GCGGGCAACGGTAGCTGCCGTTTCGACCAGA5420.10751813624650616No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT5390.10692301741119338No Hit
GGCAAACAGGCGCTGGCTTTCGCACGTGAAC5390.10692301741119338No Hit
GACCCAGCTATCGTTCAGCCGAAAGCAGCAA5160.10236043967379552No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)