FastQCFastQC Report
Sun 21 May 2023
EGAF00007951326

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007951326
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences490716
Sequences flagged as poor quality0
Sequence length44
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA43650.8895165431736484No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA39790.8108559737200335No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT24700.503346130959659No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC20590.4195909650388412No Hit
AGCGGAAGAGCCGAACAGGCGACGGCTTGCACCTGGTTTGCGGG17450.3556028334107712No Hit
GTTGCGCGGCGGACGAGTACGTACCGGAACGCGCAGACCGCTCA17370.35397256254126624No Hit
AGAACTGAGCCTTCACTGGTTCAGCTCCCTCGGTCGAGTACGCT15230.31036281678200833No Hit
GGCGCCCGCGGTTCAACCAATGGTAACACAAGAACTGAGCCTTC14860.30282281401054784No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA13750.28020280569616646No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA12220.2490238753168839No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT11550.23537035678477977No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT11450.2333325181978986No Hit
GCTAGTTATAGCGGGGCCGAGGTTACTAACCAAAGTGTCGGCAC10870.22151305439398755No Hit
GGTAGAAGCGGCGCGGGAGCTACCAGACAGGCGACCCGGTGCGG10530.21458440319859143No Hit
CGAGATGTTTCCATCCAGAGGTCTCCGCAGCGGAAAAAATTGGC9850.2007271008077992No Hit
ACCCTGAACTGGGGTAGAGGTTACAGTTTGCGCACGAGCAGAGG9500.19359466575371498No Hit
GGCGATCAGGTCGGACAGGTTTTTCTTGATAACTTCGCCAGACG9020.18381304053668518No Hit
CTGTGCGCCCTACGCGGAGGTGGCCTATTCATCGGTGCAGGATT8770.17871844406948215No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA8510.173420063743591No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT7980.1626195192331206No Hit
ACGAACAATCACCTTAATTGCTTTGCCGTTAACTTTAACTACGT7210.14692816211413526No Hit
AACCCTCTTAACCGCTGTATCGGCGAGAAGCTCTTCTGAGGGGC7030.14326005265774908No Hit
ATCGCCCGGAACTACCTGGCTAGTGTGACCGGTCTCGGCAGCGG6920.14101843021217975No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA6380.1300141018430212No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT6260.12756869553876377No Hit
GCTCAGCTGAGACGGCTGCATGTTTTTGCCGGCCAGGTGTGCTT6180.12593842466925878No Hit
ATACGGATAAGAACTACGGGCAGTGGCTGTAACCCTCTTAACCG6090.1241043699410657No Hit
AGAGGACGGACGGCTACCCTGTTTGCCGTTCCAGTAGGCCTGTG6020.12267788293024887No Hit
TTTCAGGATAGACGGGCTTGCAACCGGCGGAGCGTTGGATACAG5940.1210476120607439No Hit
CGGCGGAACCGGGACAGTAGCCTGCGCGGTGGTCACCGGTGGGG5790.11799085418042207No Hit
CGGTTCGCCATCCGGACCGCCTTCCTGGTGATACTCGAAGTAGT5760.1173795026043577No Hit
CTGGGAGGTCAGGAACACCAGGTAGCTGCGCTGAGCTTCACGGA5470.1114697707024022No Hit
CATGGTGTCGCCCGGCACTACCTGGGAAGTGTGGCCGGTCTCAG5290.10780166124601603No Hit
GTCACCCGGAACGACCTGGCTGGTGTGGCCAGTTTCTGCTGCGG5210.10617139037651106No Hit
CCAAACTTCGTCGGCCGCATCACGAGAAGCGGTCTGAGTGACGG5190.10576382265913482No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)