FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007951327

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007951327
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences490716
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT96171.9597893690036599No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA33540.6834910620399579No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC26770.5455293897080999No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT25020.5098672144376789No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC18520.37740770629040016No Hit
CGTGAACGCCAGAAACGTGTTGACGATGAGG16750.34133796330260274No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA14960.30486065259742906No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA14610.29772821754334483No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT13620.27755361553322083No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA13150.2679757741748791No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG12030.2451519820018096No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA11890.24229900798017592No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG10970.22355089298086878No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10030.20439521026418542No Hit
CAGACCCCGGAACAGAGCACCCCGTCCCGCA9950.20276493939468043No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG9480.19318709803633874No Hit
GAAGAGGCTCCGGCAGTTGATCCAGTGCAGG9440.19237196260158626No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC8260.16832546727638797No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG8000.16302708695049684No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG7620.1552833003203482No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA7160.14590924282069467No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG6840.1393881593426748No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG6730.13714653689710546No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT6310.12858761483220438No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT6090.1241043699410657No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC6010.12247409907156075No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC5900.1202324766259914No Hit
GTGCCGGTCAGCGGCTCTGCTCCGGGTCGCC5730.11676815102829335No Hit
ATGGCACGTCGCCTGCCGAAACCGACTCTGC5700.116156799452229No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT5690.11595301559354086No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG5630.11473031244141214No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG5560.11330382543059529No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC5410.11024706755027348No Hit
GCTTGGGCGCTGAAAAACCCGCACCTGGCAT5110.10413355178962985No Hit
ACCGCGTCCGGTGAAGAAGTAGCAGTTCTGA4970.10128057776799615No Hit
CTGTCTGCTGAGCAGCTGAACGTCACTGACG4940.10066922619193178No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)