FastQCFastQC Report
Sat 22 Apr 2023
EGAF00007951347

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007951347
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences489842
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT35930.733501823036816No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC35830.7314603484388843No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG30610.6248953744268559No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT22100.4511658861428788No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA17930.3660363954091319No Hit
GCACGTCGTCAGGAAGCAGAACGCCAGGCCG16820.3433760273720914No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG15630.3190824796567056No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG15150.30928340158663403No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13490.27539492326096987No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC11900.24293547715385777No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT11590.23660690590026986No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC11400.2327281041641999No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG11210.22884930242812987No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG11180.22823686004875043No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG10210.20843455644881412No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG9860.2012893953560536No Hit
TCTGGCTACGAAATTGACCACATCGACCTGG9280.18944884268805043No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT9120.18618248333135992No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA8690.1774041425602541No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG8610.17577096288190888No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC8560.17475022558294306No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG8220.1678092119499757No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC7940.1620930830757673No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG7410.15127326770672994No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA7080.14453640153355574No Hit
ACCGCGTCCGGTGAAGAAGTAGCAGTTCTGA6860.14004515741810625No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC6740.13759538790058837No Hit
ACCGCATCCGGTGAAGAAGTTGCGGTACTGA6340.12942948950886204No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC6130.12514239285320572No Hit
GAAGAGGCTCCGGCAGTTGATCCAGTGCAGG6050.12350921317486045No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG6020.12289677079548099No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC5440.11105621812747785No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG5430.11085207066768468No Hit
CCACGTCGCCCGCCGCCGGGCCGTCGTCCTT5430.11085207066768468No Hit
ACCGCATCCGGTGAAGAAGTAGCTGTTCTGT5340.10901474352954625No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)