FastQCFastQC Report
Sat 22 Apr 2023
EGAF00007951475

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007951475
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences485365
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCGCCACACCCAGAAAGCACCGAAACGTA59261.220936820743152No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA27200.5604029956836607No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC24850.5119858251006975No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA23550.4852018583952284No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22920.47222193606873175No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG20310.41844797214467466No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT15990.32944279047726965No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA15310.31543271558517816No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC12520.2579502024249791No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG10430.21488982518310962No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG9660.19902547567294715No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG9350.1926385297662584No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG7870.16214601382464744No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG7770.1600857086934575No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG7620.1569952509966726No Hit
CCGACCAGCGGTCCGCTGAACATCCCGCAAC7270.14978418303750785No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG6830.14071884046027217No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC6760.13927662686843922No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC6680.13762838276348727No Hit
AACGGTGACATGGCTACCGGCTGGGTGAAGG6590.13577410814541632No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT6550.13494998609294037No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT6520.13433189455358338No Hit
AGCCCGGCTAAATCTGCGCCGCCGTCTCCGG6290.12959319275184655No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG6240.1285630401862516No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC5770.11887960606965893No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG5650.116407239912231No Hit
TCTGCGTCCGCCTCCGTTCTGTCTCCGACCG5630.115995178885993No Hit
GCGGAAGTGAATGTTGACCAGGCTCACCTGG5580.11496502632039804No Hit
ACCGCGTCCGGTGAAGAAGTAGCAGTTCTGA5540.11414090426792207No Hit
ACCGCATCCGGTGAAGAAGTTGCGGTACTGA5450.11228662964985114No Hit
TCTGGCGCTGGCACCTCTTCTGCCTCTTCCG5440.11208059913673214No Hit
TTCAAAGCATACGCGCACATGTATGACGAAA5280.10878411092682826No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG5270.10857808041370928No Hit
TCTAAGTTCGGCGAGAAAGAAAAAGTGGCAG5020.10342731758573445No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC4950.1019851039939015No Hit
CAGGGCGCGCTGACCCTGTCCCTGCCGAAAC4870.10033685988894954No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)