FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007951528

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007951528
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences472220
Sequences flagged as poor quality0
Sequence length44
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AACCGCCGGCGGGCCGGTATCGCTTGGGGATACGGTTGGCGGTG47080.996992927025539No Hit
CGGCGGCGGTTCCTGATAACCCTGGTACGGGGCTTGCGGAGCCG31560.6683325568590911No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC29070.6156028969548092No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT25310.5359789928423193No Hit
GTGCTGGGAACGTGGGGTCCACGGGCCTGCATAACCCGGGTAGG25070.5308966159840752No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA24540.5196730337554529No Hit
TGGCGGCGGTTCCTGGTAGCCCTGATACGGTGCCTGCGGCGCCG24360.51586125111177No Hit
GTTCTGGGACGCAGCCATGTAGTCCTGCGGTGCCGCACGACGCT23980.5078141544195502No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA22450.47541400194824446No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA17320.36677819660327815No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT16300.3451780949557409No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA15670.3318368557028504No Hit
GTGCTGGCTACGCGGGGTCCACGGACCAGCATAGCCCGGGTAGG15260.3231544619033501No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT14380.3045190800897887No Hit
AGGGTGGGTCTTCGGGGTCGGCCACGGCAGTTTACGCGGTTTTT14260.30197789166066663No Hit
ACCCTGAACTGGGGTAGAGGTTACAGTTTGCGCACGAGCAGAGG12560.2659777222481047No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT12550.26576595654567786No Hit
GGTAGAAGCGGCGCGGGAGCTACCAGACAGGCGACCCGGTGCGG10950.23188344415738427No Hit
CGGATGAGTTTTCGGAGTCGGCCACGGCAGTTTACGCGGCTTCT10390.22002456482148153No Hit
GGCAATATTTAAATATGCTCGTCAGGTGGACGGACCTTCGCCCG10200.21600101647537162No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG10160.2151539536656643No Hit
CGGGTCCTGGGACCACGGAGCCCACGGATGACGGTAGCACGGGT9380.1986362288763712No Hit
CGGTTCGCCATCCGGACCGCCTTCCTGGTGATACTCGAAGTAGT9320.19736563466181017No Hit
ACCCTGACCGCGCGGGCCGGTGGACGGGCCTTCGCCCGGGTCAT9290.19673033755452968No Hit
AACGGTTACGCTGCTCTGTTCAGACGGACCCGGGCGTTCCGGGG9250.19588327474482234No Hit
GCGGACGGACATCTGAGACGGGGAACGCGGTGGGTTCGGGGTCC9080.19228325780356614No Hit
AACGCAGGCACCGCGACGACGACGGGACGGTGGTGGGGTCGGTT8980.19016560077929778No Hit
ACGACGACCGCCGTCGCCCTGACCACGTGGGCCGGTGGACGGAC8960.18974206937444413No Hit
GGTGGACGGGCCTTCGCCCGGGTCGTCAGCCGGACCCTGCTCGA8810.1865655838380416No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG8780.1859302867307611No Hit
CACAGCCGGCGGACCGGTATCAGACGGAGAGACGGTCGGTGGCG8330.17640083012155353No Hit
CGGGTGGGTTTTCGGGGTCGGCCAAGGCAGTTTACGCGGCTTTT8140.17237728177544365No Hit
CGGAGCCTGCGGTGGCGGCGGCTCTTGATAACCCTGATACGGCG7890.16708313921477277No Hit
TGGGATCTGCAGAGTCTGGTTCGGCATGATCAGATAACCACGCA7320.15501249417644317No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT7200.15247130574732115No Hit
TTTGTCGATGGTCTGCTCCAGGTTACCAAAGAAGCCTGCTTTTG7140.15120071153276016No Hit
CGGCGGAACCGGGACAGTAGCCTGCGCGGTGGTCACCGGTGGGG6720.1423065520308331No Hit
TGGGGTACCGGATACCTGTTTTTCCAGGCGACCCTGAACCGGAG6610.13997712930413791No Hit
AACGGTGACGCTAGACTGTTCGGAAGGACCCGGACGTTCTGGAG6490.13743594087501587No Hit
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA6460.13680064376773537No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA6400.13553004955317438No Hit
GTGCTGGGAACGCGGGGTCCAAGGGCCAGCGTAGCCCGGATAGC6300.13341239252890602No Hit
TGGTGCCTGAGACTCGTTCAGTTTCTTAGCTTCGCCCAGGACGT6190.13108296980221085No Hit
GAAGATGTAATCCCAGGACTCATCCAGGTCAGCCGGATCGATAG6160.13044767269493032No Hit
TGTGACATTTAAATTGGAGTATCAGGTAGACGGGCCTTCGCCCG6010.1272711871585278No Hit
CGGGCCTTCGCCCGGGTCATCAGCCGGGCCCTGTTCGATCGCAC5750.12176527889543011No Hit
CGGTGCCTGTGGCGGAGGCGGTTCCTGATAGCCCTGGTATGGTG5720.1211299817881496No Hit
GGTGGACGGACCTTCGCCCGGGTCATCCGCCGGGCCCTGTTCGA5720.1211299817881496No Hit
GGCCGGGTAGGTTGGAGTGGTGGAAGCTGCACGGGAAGAGCCGG5380.11392994790563721No Hit
GTCAGACGGACGAGTTGGCAGGGTTGCGCCGGTTTCGTTTGCGG5350.1132946507983567No Hit
CGGCGCACCGTGGTTGATCGGCTGGGTCAGCGGCAGGTCGAAGT5250.11117699377408835No Hit
CGGACGAGTCGGCAGGGTAGCACCGGTTTCGTTCGCGGTCAGTG5170.10948286815467367No Hit
ATCGCCCGGAACTACCTGGCTAGTGTGACCGGTCTCGGCAGCGG4820.10207106856973444No Hit
GCTCAGCTGAGACGGCTGCATGTTTTTGCCGGCCAGGTGTGCTT4770.10101224005760027No Hit
AAAGCCATCCTGTTTAGCTTTATTGATGATGTCCTTGATGGAGT4770.10101224005760027No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)