FastQCFastQC Report
Sun 21 May 2023
EGAF00007951559

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007951559
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences474317
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG68821.4509283875551582No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT33070.697213045284061No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA31540.6649561369295218No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT24300.5123156032779765No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC18870.3978352030393176No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA13700.28883636892626663No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG12810.2700725464193778No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA12730.26838591068842144No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA12580.2652234686928784No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC12210.25742277843720546No Hit
GATGGTTACATCTTCAACCCGAAAGACATCG11940.251730382845228No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG10940.23064743620827421No Hit
GGTTACCAGGAACCGCCGGCACCACAGGCGC10840.22853914154457883No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG10110.21314859049960258No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG9400.1981796983873654No Hit
GAAGAGGCTCCGGCAGTTGATCCAGTGCAGG9280.19564974479093097No Hit
GGCTACCAGGAACCACCGGCACCACAAGCCC9030.19037900813169253No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG8900.18763822506888853No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC8710.1836324652078673No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT8600.1813133410778024No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG8520.1796267053468461No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC8090.170561038292956No Hit
AGCCTGGGTTCCCTGAGCTCTAACCCAAGCT7510.1583329292435228No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA7460.1572787819116751No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC7380.15559214618071882No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG7000.14758062645867637No Hit
GAACTGCTGATGAAGGACCCGAACTACCAGC6960.14673730859319822No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG6630.13977993620300347No Hit
CAGGGCGCGCTGACCCTGTCCCTGCCGAAAC6500.1370391531401995No Hit
AAAGTGTACAGCCAGAACGGTCTGGTCCTGC6360.13408754061102596No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT6180.13029261021637428No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA6040.12734099768720075No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG5660.11932947796515832No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA5580.11764284223420203No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC5440.11469122970502849No Hit
ATTGAGCAGAAACAGCTGCTGCAGGGTGATG5360.1130045939740722No Hit
TACTATAACGGTAAAGTTCCGTATGATGCAC5210.10984215197852912No Hit
GTGGGCGGCTCTGATGCACAGAACACCTCTA5080.10710136891572515No Hit
CTGCACGACGACGCTAACTTTCTGGAACATG5020.10583639211750791No Hit
CAGGACTCTCTGCCGGACACTAAAGCCTCTG4880.10288477958833439No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT4820.10161980279011716No Hit
GAGAAAACGAAACAGACTCTGGCTTCTGTTT4770.10056565545826945No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)