FastQCFastQC Report
Wed 31 May 2023
EGAF00007952216

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007952216
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences179993462
Sequences flagged as poor quality0
Sequence length91
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTT7418890.4121755266866305Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA5845250.32474790667674364Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAA5612430.3118129924074687No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA5000580.2778200910430847Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC4116660.2287116406483698Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGATAGGTTTGGTCCTAGCCTT3846020.21367553894818692Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGAAAAAACCTTGTAGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCT2718750.15104715303492525No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAAAAAAAAAAAAAAAAAA2704490.15025490203638617Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2520000.14000508529582034No Hit
AAAAATTTAACACCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAG2505610.1392056118127224No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCGTGGCGGCGACGACCCATT2404260.13357485173544803Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGATAAAATTTGAAATCTG1891530.10508881705936629Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAAAAAACCTTGTAGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTA1862360.10346820263949365No Hit
AAAAAACCTTGTAGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAA1859700.10332041949390361No Hit
GCCACCAATTAAGAAAGCGTTCAAGCTCAACACCCACTACCTAAAAAATC1800320.10002141077768703No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)