FastQCFastQC Report
Wed 31 May 2023
EGAF00007953536

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007953536
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30469951
Sequences flagged as poor quality0
Sequence length8
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGT1012205933.2198072783248Illumina Paired End PCR Primer 2 (100% over 8bp)
CATCGTCG1000164332.824611368754745No Hit
GTCATATA962435931.586394740181895No Hit
TCGGACAC2354930.7728696380246887No Hit
CATCGTCA419820.13778164592388087No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)