FastQCFastQC Report
Wed 31 May 2023
EGAF00007953560

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007953560
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25823942
Sequences flagged as poor quality0
Sequence length8
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCACTCCT708695427.44334695299424No Hit
ATCTGATC667051825.83075039434336Illumina PCR Primer Index 9 (100% over 8bp)
CGTGCTAA585069222.656076287655853No Hit
GAGAAGGG578262822.39250692245204No Hit
TCACTCCA380930.14751039945799133No Hit
ATCTGATA322250.1247873000953921No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)