FastQCFastQC Report
Wed 31 May 2023
EGAF00007953564

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007953564
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25327494
Sequences flagged as poor quality0
Sequence length8
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCACTCCT695518027.460987652390717No Hit
ATCTGATC654721525.850228214445536Illumina PCR Primer Index 9 (100% over 8bp)
CGTGCTAA571454222.56260331164228No Hit
GAGAAGGG564394922.283882487544957No Hit
TCACTCCA387370.15294446422531974No Hit
ATCTGATA327520.12931401740732817No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)