FastQCFastQC Report
Wed 31 May 2023
EGAF00007954494

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007954494
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences113287145
Sequences flagged as poor quality0
Sequence length29
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTT20289241.7909569527945999Clontech SMART CDS Primer II A (100% over 26bp)
GCAGTGGTATCAACGCAGAGTACTTTTTT4069050.3591802053092608Clontech SMART CDS Primer II A (100% over 24bp)
AGCAGTGGTATCAACGCAGAGTACTTTTT1817360.16042067261912196Clontech SMART CDS Primer II A (100% over 25bp)
GTCTCGTTCGTTATCGGAATTAACCAGAC1395160.12315254303566392No Hit
AAGCCGCAGGCTCCACTCCTGGTGGTGCC1185580.10465265057213685No Hit
ACCTTTAAGTTTCAGCTTTGCAACCATAC1169600.10324207570064547No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)