FastQCFastQC Report
Mon 22 May 2023
EGAF00007954501

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007954501
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences144017619
Sequences flagged as poor quality0
Sequence length93
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT36722522.549863013635852Clontech SMART CDS Primer II A (100% over 26bp)
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT6834220.47454054909767673Clontech SMART CDS Primer II A (100% over 24bp)
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT2949000.2047666126184186Clontech SMART CDS Primer II A (100% over 25bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1468120.10194030495671506No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)