FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007954578

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007954578
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68818037
Sequences flagged as poor quality0
Sequence length91
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA30651474.453987840426196Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAAAAAAAAAAAAAAAAAA26710543.8813283790701556Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGAAAAAAAAAAAAAAAAA12542431.8225498062375711Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA10846801.576156553259431Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAGAAAAAAAAAAAAAAAAAA9759131.418106418815753Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGAGAAAAAAAAAAAAAAA5438680.7902986247631562Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAAA3995450.5805818029944678Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACATGGGGGAAAAAAAAAAAAAAAAAAAA3616800.5255598906432045Clontech SMARTer II A Oligonucleotide (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAAAAAAAAAAAAAAAAA3475990.5050986851019886Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAA3429290.49831267346378977No Hit
GTGGTATCAACGCAGAGTACATGGGGGAAAAAAAAAAAAAAAAAAAAAAA3284210.47723099105544087No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGAAAAAAAAAAAAAAAAA2905830.4222483126044412Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGAAAAAAAAAAAAAAAAA2707810.39347387952957735Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAA2486480.36131225306528286Clontech SMARTer II A Oligonucleotide (100% over 22bp)
AGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAA2377280.34544432006975145Clontech SMARTer II A Oligonucleotide (100% over 24bp)
CAGTGGTATCAACGCAGAGTACATGGGGGAAAAAAAAAAAAAAAAAAAAA2315090.3364074450423513Clontech SMARTer II A Oligonucleotide (100% over 22bp)
AGCAGTGGTATCAACGCAGAGTACATGGGGGAAAAAAAAAAAAAAAAAAA2129430.3094290527351136Clontech SMARTer II A Oligonucleotide (100% over 24bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG2111100.3067655068394351Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCAGTGGTATCAACGCAGAGTACATGGGGAGAAAAAAAAAAAAAAAAAAA1838360.26713345514345316Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GTGGTATCAACGCAGAGTACATGGGGAGAAAAAAAAAAAAAAAAAAAAAA1644800.23900710797664862No Hit
AGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAA1563390.2271773604934416Clontech SMARTer II A Oligonucleotide (100% over 21bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAGAGAAAAAAAAAAAAAAAA1530680.22242424613186804Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AGTGGTATCAACGCAGAGTACATGGGGGAAAAAAAAAAAAAAAAAAAAAA1471520.21382766265187134Clontech SMARTer II A Oligonucleotide (100% over 21bp)
AAGCAGTGGTATCAACGCAGAGTACATGGAGAGAAAAAAAAAAAAAAAAA1455010.21142858230611838Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCAGTGGTATCAACGCAGAGTACATGGGAGAAAAAAAAAAAAAAAAAAAA1281470.1862113561884946Clontech SMARTer II A Oligonucleotide (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGTCAAGTAGAAAAGGGCGCAC1251980.18192614241525082Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1206420.17530578502260968No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAA1202270.1747027454444828Clontech SMARTer II A Oligonucleotide (100% over 23bp)
CAGTGGTATCAACGCAGAGTACATGGGGAGAAAAAAAAAAAAAAAAAAAA1171610.17024751810342978Clontech SMARTer II A Oligonucleotide (100% over 22bp)
GTGGTATCAACGCAGAGTACATGGGAGAAAAAAAAAAAAAAAAAAAAAAA1096630.15935211868946508No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGGAGAAAAAAAAAAAAAAAAAA1061780.15428803934061647Clontech SMARTer II A Oligonucleotide (100% over 24bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAGAAAAAAAAAAAAAAAA1057380.15364867207706026Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAA1020890.14834628311179524No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1012020.1470573768327626No Hit
GGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAA923020.1341247208199211No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTGAAAAAAAAAAAAAAAAAA892370.12967094658628522Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGTATCAACGCAGAGTACATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAA882650.12825852617679287No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAGAAAAAAAAAAAAAAAA856720.12449061864406276Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAGGAAAAAAAAAAAAAAA830720.12071253935941242Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCAGTGGTATCAACGCAGAGTACATGGGGAGAGAAAAAAAAAAAAAAAAA825410.11994093932089345Clontech SMARTer II A Oligonucleotide (100% over 23bp)
CAGTGGTATCAACGCAGAGTACATGGGAGAAAAAAAAAAAAAAAAAAAAA796820.11578650521519526Clontech SMARTer II A Oligonucleotide (100% over 22bp)
AAGCAGTGGTATCAACGCAGAGTACATGGAGAAAAAAAAAAAAAAAAAAA781200.1135167514295707Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGTGGTATCAACGCAGAGTAC767370.11150710387162018Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AGCAGTGGTATCAACGCAGAGTACATGGGAGAAAAAAAAAAAAAAAAAAA738800.10735557598075633Clontech SMARTer II A Oligonucleotide (100% over 24bp)
AGTGGTATCAACGCAGAGTACATGGGGAGAAAAAAAAAAAAAAAAAAAAA738370.10729309236181789Clontech SMARTer II A Oligonucleotide (100% over 21bp)
CAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAA738010.10724078049479963Clontech SMARTer II A Oligonucleotide (100% over 22bp)
GTGGTATCAACGCAGAGTACATGGGGAGAGAAAAAAAAAAAAAAAAAAAA735590.10688912849984372No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAA732580.1064517431672746Clontech SMARTer II A Oligonucleotide (100% over 24bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)