FastQCFastQC Report
Mon 3 Apr 2023
EGAF00007956492

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007956492
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56234528
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGGACACATCTCGTAT7004071.245510587374362TruSeq Adapter, Index 7 (97% over 36bp)
GAAGAGCACACGTCTGAACTCCAGTCACAAGGACACATCTCGTATGCCGT3753480.6674689258528141TruSeq Adapter, Index 7 (96% over 31bp)
AAGAGCACACGTCTGAACTCCAGTCACAAGGACACATCTCGTATGCCGTC2436910.43334764008333104TruSeq Adapter, Index 7 (96% over 30bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGGACACATCGCGTAT2409230.42842539729327866TruSeq Adapter, Index 7 (97% over 36bp)
AATGATACGGCGACCACCGAGATCGGAAGAGCACACGTCTGAACTCCAGT2392510.42545213502992324Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GAAGAGCACACGTCTGAACTCCAGTCACAAGGACACATCGCGTATGCCGT2370130.4214723736989488TruSeq Adapter, Index 7 (96% over 31bp)
GGAAGAGCACACGTCTGAACTCCAGTCACAAGGACACATCTCGTATGCCG1850050.3289882685598428TruSeq Adapter, Index 7 (96% over 32bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGGACACATCTCGTATG1742050.3097829859974996TruSeq Adapter, Index 7 (97% over 35bp)
AGAGCACACGTCTGAACTCCAGTCACAAGGACACATCTCGTATGCCGTCT1575850.28022819005433813TruSeq Adapter, Index 7 (96% over 29bp)
AAGAGCACACGTCTGAACTCCAGTCACAAGGACACATCGCGTATGCCGTC1406310.2500794529652672TruSeq Adapter, Index 7 (96% over 30bp)
AGCACACGTCTGAACTCCAGTCACAAGGACACATCTCGTATGCCGTCTTC1292290.22980365372676373TruSeq Adapter, Index 7 (96% over 27bp)
GAGCACACGTCTGAACTCCAGTCACAAGGACACATCTCGTATGCCGTCTT1140790.20286291013236565TruSeq Adapter, Index 7 (96% over 28bp)
AGCACACGTCTGAACTCCAGTCACAAGGACACATCGCGTATGCCGTCTTC1037960.18457699155934945TruSeq Adapter, Index 7 (96% over 27bp)
GGAAGAGCACACGTCTGAACTCCAGTCACAAGGACACATCGCGTATGCCG928470.16510674722832208TruSeq Adapter, Index 7 (96% over 32bp)
AGAGCACACGTCTGAACTCCAGTCACAAGGACACATCGCGTATGCCGTCT857540.1524935000788128TruSeq Adapter, Index 7 (96% over 29bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACAAGGACACATCTCGTATGCC791570.14076227331364816TruSeq Adapter, Index 7 (96% over 33bp)
GAGCACACGTCTGAACTCCAGTCACAAGGACACATCGCGTATGCCGTCTT764730.1359894049435251TruSeq Adapter, Index 7 (96% over 28bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGGACACATCGCGTATG670660.1192612481783434TruSeq Adapter, Index 7 (97% over 35bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACAAGGACACATCGCGTATGCC605210.10762249129218263TruSeq Adapter, Index 7 (96% over 33bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)