FastQCFastQC Report
Sun 2 Apr 2023
EGAF00007956534

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007956534
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38400377
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCACACGTCTGAACTCCAG1476620.384532683103606Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AATGATACGGCGACCACCGAGATCGGAAGAGCACACGTCTGAACTCCAGT678860.17678472271248796Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAGCCAATCTCGTAT678840.17677951443028803TruSeq Adapter, Index 7 (97% over 37bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)