FastQCFastQC Report
Mon 3 Apr 2023
EGAF00007958366

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007958366
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52797961
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCGTATCGAAGGCCTTTTTGGACAGGTGGTGTGTGGTGGCCTTGGTATG2178560.4126219950046935No Hit
CTCCGATTATGATGGGTATTACTATGAAGAAGATTATTACAAATGCATGG1176960.22291769941646042No Hit
CTGCTGTTAGAGAAATGAATGAGCCTACAGATGATAGGATGTTTCATGTG856230.16217103535494487No Hit
GTTTGGATGTAAAGTGAAATATTAGTTGGCGGATGAAGCAGATAGTGAGG792150.15003420302537818No Hit
CCGGAGTAGTAAGTTACAATATGGGAGATTATTCCGAAGCCTGGTAGGAT758590.14367789695514946No Hit
GTTGGTTAGTAGGCCTAGTATGAGGAGCGTTATGGAGTGGAAGTGAAATC730630.13838223790498275No Hit
GTGGGCTACAACGTAGTACGTGTCGTGTAGTACGATGTCTAGTGATGAGT722150.13677611527460312No Hit
GTTGTTTTCTATTAGACTATGGTGAGCTCAGGTGATTGATACTCCTGATG692820.13122097650702838No Hit
CTAGTGATGAGTTTGCTAATACAATGCCAGTCAGGCCACCTACGGTGAAA691790.1310258932158384No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTCGATATCATCTCGTATG662080.1253987819719023TruSeq Adapter, Index 10 (97% over 35bp)
CTTCCGTGGAGTGTGGCGAGTCAGCTAAATACTTTGACGCCGGTGGGGAT647230.12258617335620214No Hit
CTAGATTTTATGTATACGGGTTCTTCGAATGTGTGGTAGGGTGGGGGGCA641830.12156340658685665No Hit
CTCGTGTTACATCGCGCCATCATTGGTATATGGTTAGTGTGTTGGTTAGT624150.11821479242351803No Hit
CTCAGGAGTTTGATAGTTCTTGGGCAGTGAGAGTGAGTAGTAGAATGTTT587820.11133384488086576No Hit
GCCGTAGATGCCGTCGGAAATGGTGAAGGGAGACTCGAAGTACTCTGAGG575440.10898905736151439No Hit
GTGGTGTATGCATCGGGGTAGTCCGAGTAACGTCGGGGCATTCCGGATAG564570.10693026573507262No Hit
GTCGTTACCTAGAAGGTTGCCTGGCTGGCCCAGCTCGGCTCGAATAAGGA553470.10482791182030685No Hit
GGTGTATGCATCGGGGTAGTCCGAGTAACGTCGGGGCATTCCGGATAGGC537310.10176718756241362No Hit
GGCCAATTGATTTGATGGTAAGGGAGGGATCGTTGACCTCGTCTGTTATG536780.1016668048980149No Hit
CCCCGGTCGTGTAGCGGTGAAAGTGGTTTGGTTTAGACGTCCGGGAATTG530040.10039024044886885No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)