FastQCFastQC Report
Tue 4 Apr 2023
EGAF00007958367

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007958367
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53703830
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTAGCGCTATCTCGTAT3528450.657020179007717TruSeq Adapter, Index 3 (97% over 37bp)
CCCGTATCGAAGGCCTTTTTGGACAGGTGGTGTGTGGTGGCCTTGGTATG1905140.3547493726238892No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCTAGCGCTATCTCGTATG1222840.22770070589006405TruSeq Adapter, Index 3 (97% over 36bp)
CTGCTGTTAGAGAAATGAATGAGCCTACAGATGATAGGATGTTTCATGTG988960.1841507393420544No Hit
CTCCGATTATGATGGGTATTACTATGAAGAAGATTATTACAAATGCATGG865730.16120451744316933No Hit
GTTTGGATGTAAAGTGAAATATTAGTTGGCGGATGAAGCAGATAGTGAGG801970.1493319936399322No Hit
CTAGATTTTATGTATACGGGTTCTTCGAATGTGTGGTAGGGTGGGGGGCA797460.148492202511441No Hit
GTGGGCTACAACGTAGTACGTGTCGTGTAGTACGATGTCTAGTGATGAGT789790.14706399897363No Hit
CTAGTGATGAGTTTGCTAATACAATGCCAGTCAGGCCACCTACGGTGAAA703470.13099065746335037No Hit
GTTGTTTTCTATTAGACTATGGTGAGCTCAGGTGATTGATACTCCTGATG689560.12840052562359147No Hit
TGTAAAGTGAAATATTAGTTGGCGGATGAAGCAGATAGTGAGGAAAGTTG657880.12250150501370201No Hit
TGCTGTTAGAGAAATGAATGAGCCTACAGATGATAGGATGTTTCATGTGG618960.11525434964321912No Hit
CCCCGGTCGTGTAGCGGTGAAAGTGGTTTGGTTTAGACGTCCGGGAATTG612200.11399559398277553No Hit
CTCATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAAAA608400.11328800943992262No Hit
CTTCCGTGGAGTGTGGCGAGTCAGCTAAATACTTTGACGCCGGTGGGGAT594010.11060849850001388No Hit
GTTGGTTAGTAGGCCTAGTATGAGGAGCGTTATGGAGTGGAAGTGAAATC589600.10978732801738722No Hit
GGTGTATGCATCGGGGTAGTCCGAGTAACGTCGGGGCATTCCGGATAGGC577880.10760498832206195No Hit
GCCGTAGATGCCGTCGGAAATGGTGAAGGGAGACTCGAAGTACTCTGAGG577670.10756588496574639No Hit
GGCCAATTGATTTGATGGTAAGGGAGGGATCGTTGACCTCGTCTGTTATG571080.10633878440327255No Hit
GTGGTGTATGCATCGGGGTAGTCCGAGTAACGTCGGGGCATTCCGGATAG561110.1044823060105769No Hit
GGCGGATGAAGCAGATAGTGAGGAAAGTTGAGCCAATAATGACGTGAAGT555320.10340417061501946No Hit
CGGATGTGTTTAGGAGTGGGACTTCTAGGGGATTTAGCGGGGTGATGCCT540870.10071348728759195No Hit
GTGGGAAGAAAGTTAGATTTACGCCGATGAATATGATAGTGAAATGGATT540220.10059245308947239No Hit
CTCAGGGTTTGTTATAATTTTTTATTTTTATGGGCTTTGGTGAGGGAGGT537760.10013438520120445No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)