FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007958380

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007958380
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences529561
Sequences flagged as poor quality0
Sequence length44
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT29210.5515889576460502No Hit
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA28240.533271898799194No Hit
GGCGCCCGCGGTTCAACCAATGGTAACACAAGAACTGAGCCTTC27310.515710182585198No Hit
AGAACTGAGCCTTCACTGGTTCAGCTCCCTCGGTCGAGTACGCT27150.5126888120537578No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA25480.48115325713185075No Hit
GGTAGAAGCGGCGCGGGAGCTACCAGACAGGCGACCCGGTGCGG21850.4126059131998014No Hit
CGAGATGTTTCCATCCAGAGGTCTCCGCAGCGGAAAAAATTGGC18340.3463245971663321No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG17710.33442795069878634No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG15420.2911845849675486No Hit
TTTCAGGGACGGTACGCCGACACGAGAACCGCGACCTTTAACTG12760.2409542998823554No Hit
AACCCTCTTAACCGCTGTATCGGCGAGAAGCTCTTCTGAGGGGC12100.22849114644016458No Hit
GAAGATGTAATCCCAGGACTCATCCAGGTCAGCCGGATCGATAG11430.21583915733975875No Hit
ATACGGATAAGAACTACGGGCAGTGGCTGTAACCCTCTTAACCG11340.21413963641582368No Hit
AGACGCTAGATGAGGAGCGTACGGAGCTGAACACAGATTTAAAT11080.20922990930223334No Hit
TGCGTCTTCGAAATCCTCGTCATCGTCATCTTCTTCTTCACGCA11040.2084745666693733No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT10390.1962002488853975No Hit
CGGACGAGTCGGCAGGGTAGCACCGGTTTCGTTCGCGGTCAGTG9980.18845798689858204No Hit
GTCAGACGGACGAGTTGGCAGGGTTGCGCCGGTTTCGTTTGCGG9790.1848701093924968No Hit
GGTTGCCAGCGGACCAATGATAGTCGGCGGGGTTTTCGGACGGC9450.1784496970131864No Hit
TTTCTGCAGGATGTTCTGTACCGCGTCGGACGGGGTTTTGGCAC8160.15408989710344984No Hit
ACCAATGATGGTCGGCGGGGTTTTCGGACGAGAGGTTTCCGGAC8140.15371222578701982No Hit
TGCATCTTCGAAGTCTTCATCATCGTCGTCGTCTTCTTCTTCAC7900.14918016998985953No Hit
GCTAGTTATAGCGGGGCCGAGGTTACTAACCAAAGTGTCGGCAC7810.1474806490659244No Hit
TGACCTATTTAAATACGAGCCTCAGTCAGACGGACGAGTCGGCA7770.14672530643306436No Hit
AGAGGACGGACGGCTACCCTGTTTGCCGTTCCAGTAGGCCTGTG7670.14483694985091425No Hit
GGCCGGGTAGGTTGGAGTGGTGGAAGCTGCACGGGAAGAGCCGG7610.1437039359016242No Hit
GTTGCGCGGCGGACGAGTACGTACCGGAACGCGCAGACCGCTCA7490.14143790800304404No Hit
GTGGGAGTTACGGCATACCGGTGCCGCGTTAGATGGACCCGGGG7000.13218496075050845No Hit
ATCGCCCGGAACTACCTGGCTAGTGTGACCGGTCTCGGCAGCGG6920.13067427548478835No Hit
CGGCGGCGGTTCCTGATAACCCTGGTACGGGGCTTGCGGAGCCG6790.1282194119279932No Hit
CCCATGACGGATTCCTGTCACCGAAGGATGCGTACTCCTGGCGA6530.12330968481440287No Hit
TGCTGGTTTCGCGGCCGGTTTTGCGGTCGGTTTCGCAGCCGGTT6510.12293201349797285No Hit
GCTCAGCTGAGACGGCTGCATGTTTTTGCCGGCCAGGTGTGCTT6310.1191553003336726No Hit
CGTACTCCTGGCGACAGAGAAAATGGCGGTGACGTTATTTAAAT6210.1172669437515225No Hit
GAAGCCACCTTTGGAGCGGGTTTTAGAATCACGCTTACGGCGCA5990.11311255927079222No Hit
CGGCGAAGCGACCGTTGAAAAAGGTGGATGCAACTGATTTAAAT5960.11254605229614718No Hit
CGGTTTATCTTTCTCGACGTCTTCCGCCAGCGGGAATGCCGGGC5700.10763632518255688No Hit
GGACTGGTCCGGCAGGGTATCCGGGAAAGCCGCACGTGCCGCAC5630.1063144755750518No Hit
GTCGCCCGGCAGCGGTGCAGTCGGAGCCCACGGGGAGTTACCGT5630.1063144755750518No Hit
CTGTGCGCCCTACGCGGAGGTGGCCTATTCATCGGTGCAGGATT5610.10593680425862177No Hit
GTACTCCAGTCGGTGTTTCCGCCCTCTACGCGGCGAAGCGACCG5600.10574796860040674No Hit
CATGGTGTCGCCCGGCACTACCTGGGAAGTGTGGCCGGTCTCAG5470.1032931050436116No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)