FastQCFastQC Report
Wed 31 May 2023
EGAF00007958381

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007958381
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences529561
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT62911.187965125830641No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA58271.1003453804188752No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG42050.7940539427941257No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT29500.5570651917342856No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA25590.4832304493722159No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA24040.45396092234888896No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG22150.41827098294625165No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC19660.3712509040507137No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG19010.35897658626673795No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG18970.35822124363387786No Hit
ACCGCGTCCGGTGAAGAAGTAGCAGTTCTGA14780.27909910284178785No Hit
ACCGCATCCGGTGAAGAAGTAGCTGTTCTGT13290.250962589767751No Hit
ACCGCATCCGGTGAAGAAGTTGCGGTACTGA12900.24359799909736557No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA12370.2335897092119699No Hit
TCTGCGTCCGCCTCCGTTCTGTCTCCGACCG11990.22641395419979948No Hit
GCCGTCCGTTATTTCCGCGGCCGCGGCTCCG11930.22528094025050938No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC11410.21546148602332874No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA10590.19997696204969778No Hit
GATGGCGAACACCTGGAAGGTGGCGACGGCA10400.19638908454361254No Hit
GTGCGTTATTTCCGTGGCCGCGGCAGCGGTT10140.19147935743002223No Hit
CTGCCGCAGTCTCCAGGCCCAGCGTTCCCGC9880.18656963031643192No Hit
ATTGCTGCCGTTGGCTCTCCAGTGAAGTCTA8310.15692243197667502No Hit
AGCCCGGCTAAATCTGCGCCGCCGTCTCCGG8310.15692243197667502No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC7950.1501243482809346No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT7790.14710297774949438No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC7500.14162674366125905No Hit
CGCGGCCGTGGTAAAGGTAAGTCTCGTGATA7370.1391718801044639No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG7070.1335068103580135No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG6770.12784174061156317No Hit
CGTAAAGCTAAACCGGCAACTAAGCCGGCAG6520.12312084915618786No Hit
GGCGCACCGTCCGGCGGCCCGAGCGACGTTA5800.10952468176470699No Hit
CACACCCCTCCGATGCCGTCTATCGGTCTGG5710.10782516084077189No Hit
GGCGAGTTCCGCCTGCACAACCCGATCAAAG5630.1063144755750518No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG5610.10593680425862177No Hit
TTCGAACAGCCGACCCTGACCTTCGGCGCGG5530.10442611899290166No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG5370.10140474846146148No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)