FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007958388

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007958388
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences480682
Sequences flagged as poor quality0
Sequence length44
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC26510.5515080656234267No Hit
GGCATCGGTGAAGCCAGTGCCGAATGCGTCACCCAGACCTTTGA22690.4720376465105829No Hit
GGTAGAAGCGGCGCGGGAGCTACCAGACAGGCGACCCGGTGCGG19400.4035932279552802No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT18650.3879903969776276No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA17270.35928118797874686No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT17150.35678473502232244No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA15440.32121028039327454No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT10890.22655310579551552No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT10540.21927178467261102No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA10480.21802355819439878No Hit
GGCAATATTTAAATATGCTCGTCAGGTGGACGGACCTTCGCCCG9450.19659567031842257No Hit
ACCACCTTTTTTACGGCGGCCGCCATCACCCTGACCACGTGGAC9360.19472333060110428No Hit
GGCCGGGTAGGTTGGAGTGGTGGAAGCTGCACGGGAAGAGCCGG9010.18744200947819972No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA7970.16580608385585482No Hit
GCTCAGCTGAGACGGCTGCATGTTTTTGCCGGCCAGGTGTGCTT7500.15602830977652585No Hit
ACCCTGACCGCGCGGGCCGGTGGACGGGCCTTCGCCCGGGTCAT7150.14874698865362132No Hit
CAGCGCTTCCACCTCGCGAGAAACTGCATCAGTGAAGCCGGTGC7020.14604249795082822No Hit
CTGACCACGGTGTTTACCGAACCAACCACCTTTTTTGCGACGGC6470.13460042190054963No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT6350.1321039689441252No Hit
CGGGCAGGACAGACCAGAGGTCAGCTTACAGCGGCATACGTTTG6270.1304396669731756No Hit
CAGAGCGAAAGTAATGTTTTTGCCATCACGCTTAACCTTCAGGT6220.1293994782413321No Hit
AGACGCTAGATGAGGAGCGTACGGAGCTGAACACAGATTTAAAT6110.1271110630312764No Hit
GCGAGTCTGATCGGTGATAACATAACGAGTTTCGATCATATCTT5910.12295030810390237No Hit
AGAGGACGGACGGCTACCCTGTTTGCCGTTCCAGTAGGCCTGTG5540.11525291148826043No Hit
ACGAACAATCACCTTAATTGCTTTGCCGTTAACTTTAACTACGT5460.11358860951731081No Hit
GGTGGACGGACCTTCGCCCGGGTCATCCGCCGGGCCCTGTTCGA5460.11358860951731081No Hit
TGCGTCTTCGAAATCCTCGTCATCGTCATCTTCTTCTTCACGCA5420.11275645853183601No Hit
TGGGATCTGCAGAGTCTGGTTCGGCATGATCAGATAACCACGCA5160.10734747712624979No Hit
GTTGACCCATTCGCCGTTAGCGTTAACACCATAGCCGTCAACGG5080.10568317515530018No Hit
GGTGGACGGGCCTTCGCCCGGGTCGTCAGCCGGACCCTGCTCGA5050.10505906191619407No Hit
CGGGCCTTCGCCCGGGTCATCAGCCGGGCCCTGTTCGATCGCAC5020.10443494867708797No Hit
AGCGGAAGAGCCGAACAGGCGACGGCTTGCACCTGGTTTGCGGG4970.10339475994524447No Hit
TTTGGCAACAACCTGTACTGGCGGCACCGGCGGCGGGCTCAGGT4920.10235457121340095No Hit
AATACCGCGGCCCTGGTTGTACACGCCGTGGTGTTCATGTGCGT4900.10193849572066355No Hit
GTAATCGTTGCGTGGCCAGATGTACGGAGAGTTGTGGTCGTATG4870.10131438248155746No Hit
TGTGACATTTAAATTGGAGTATCAGGTAGACGGGCCTTCGCCCG4840.10069026924245134No Hit
GGACTGGTCCGGCAGGGTATCCGGGAAAGCCGCACGTGCCGCAC4830.10048223149608265No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)