FastQCFastQC Report
Sun 21 May 2023
EGAF00007958391

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007958391
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences477166
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC37780.7917580045518751No Hit
AGCGGTGACTGTGGCGGCATCCTGCGCTGCC23390.4901858053591413No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG19950.41809349366886994No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG19100.4002799864198204No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT17430.36528168394227584No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA17150.35941370508376536No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT17130.35899456373672894No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA12240.2565145043863142No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG11370.23828185579022812No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG11170.23409044231986353No Hit
AGCCCGGCTAAATCTGCGCCGCCGTCTCCGG9940.20831324947712115No Hit
ACCGCGTCCGGTGAAGAAGTAGCAGTTCTGA9400.19699643310713671No Hit
GCCGAAGCTGATTATTTCGAATACCACCAGG9390.1967868624336185No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC9370.19636772108658201No Hit
ACCGCATCCGGTGAAGAAGTAGCTGTTCTGT8810.18463176336956114No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8780.1840030513490064No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG8350.1749915123877225No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG8320.17436280036716784No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC8120.17017138689680322No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT7650.16032156524144636No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG6780.1420889166453603No Hit
ACCGCATCCGGTGAAGAAGTTGCGGTACTGA6730.14104106327776916No Hit
GGTGACGTTGCTAAAGCGATCAATGACGCGG6580.1378975031749957No Hit
ACCCAGAACCCAGTGGAAAACTACATCGACT6530.13684964980740455No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC6450.1351730844192587No Hit
TCCACCGGTGGTAACGGCCTGGTTGGTTTCG6320.1324486656635217No Hit
CTGATCCTGGGCCTGGTTCTGGAACAGGTGC6200.12993381758130293No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC6200.12993381758130293No Hit
GCACCGAAGCCTTCCAAACAGAGCAAGAAGA5840.12238927333464665No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT5570.11673086514965442No Hit
GGCGAAGGTGTAGTTGGCTTCGCGGATGTTC5490.11505429976150856No Hit
GCCGTCCGTTATTTCCGCGGCCGCGGCTCCG5420.11358730504688096No Hit
GTGGGCGGCTCTGATGCACAGAACACCTCTA5410.11337773437336274No Hit
CCACGTCGCCCGCCGCCGGGCCGTCGTCCTT5350.11212031033225335No Hit
TACGACGACTTCCATACCGATGAAGATAAAC5310.11128202763818042No Hit
GTGCGTTATTTCCGTGGCCGCGGCAGCGGTT5160.10813846753540697No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA5030.10541404877966996No Hit
CTGGACACCAACTCCGTTTATGAACCGTACT4950.10373748339152414No Hit
ACTCGTCTGGAAAACATCAAAACCGACCGTA4910.10289920069745119No Hit
CGTGAACGCCAGAAACGTGTTGACGATGAGG4890.10248005935041475No Hit
AACGGTGACATGGCTACCGGCTGGGTGAAGG4860.10185134732986006No Hit
TCCTACACCAACGGCAAACTGACTATCACCC4860.10185134732986006No Hit
GGCGAGTTCCGCCTGCACAACCCGATCAAAG4780.10017478194171421No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)