FastQCFastQC Report
Wed 31 May 2023
EGAF00007958400

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007958400
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences414414
Sequences flagged as poor quality0
Sequence length44
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AACCGCCGGCGGGCCGGTATCGCTTGGGGATACGGTTGGCGGTG31920.7702442485051181No Hit
CGGCGCACCGTGGTTGATCGGCTGGGTCAGCGGCAGGTCGAAGT26410.6372854198941155No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT26300.6346310694136781No Hit
GCGGACGGACATCTGAGACGGGGAACGCGGTGGGTTCGGGGTCC24160.5829918873397134No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC23600.5694788303483956No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA17500.4222830309786832No Hit
CTGAACAACATCGGTACCCTGAGAAGGCGGTGCGCCCTGAAACA16030.38681125637647373No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA15590.376193854454724No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA15550.3752286360982013No Hit
AGAACTGAGCCTTCACTGGTTCAGCTCCCTCGGTCGAGTACGCT14650.35351122307644045No Hit
GGCGCCCGCGGTTCAACCAATGGTAACACAAGAACTGAGCCTTC13330.3216590173111912No Hit
ATCGCCCGGAACTACCTGGCTAGTGTGACCGGTCTCGGCAGCGG13130.3168329255285777No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT13110.31635031635031635No Hit
CGGCGGCGGTTCCTGATAACCCTGGTACGGGGCTTGCGGAGCCG12840.3098350924437881No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG12550.3028372593589985No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT12510.3018720410024758No Hit
GACCGGCCATTCCGGGGTGCGCGGACGTGGACGACCGTGAATAG12380.298735081343777No Hit
CGCCTGCGGGCACGGACGACCGTGAATGGACAGATCCAGTGCTT12290.2965633400416009No Hit
TGGCGGCGGTTCCTGGTAGCCCTGATACGGTGCCTGCGGCGCCG11300.272674185717664No Hit
CATGGTGTCGCCCGGCACTACCTGGGAAGTGTGGCCGGTCTCAG10390.2507154681067725No Hit
GTGCTGGGAACGTGGGGTCCACGGGCCTGCATAACCCGGGTAGG10120.2442002442002442No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA10110.2439589396111135No Hit
ACCCTGACCGCGCGGGCCGGTGGACGGGCCTTCGCCCGGGTCAT10080.2432350258437215No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG10060.24275241666546016No Hit
CGAGATGTTTCCATCCAGAGGTCTCCGCAGCGGAAAAAATTGGC9230.22272413576761405No Hit
GATTTCCGGGGTTTTGACAGAACGCAGCAGGTCAGCGATGGCCG9210.2222415265893527No Hit
GCTCGGCTCCTGCTGCATCTGGGTGATTTCGCGGCCAGCACGCA9120.2200697852871766No Hit
GTCACCCGGAACGACCTGGCTGGTGTGGCCAGTTTCTGCTGCGG9060.21862195775239254No Hit
GAAGATGTAATCCCAGGACTCATCCAGGTCAGCCGGATCGATAG8810.21258934302412566No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT8490.204867596171944No Hit
CATGGTGTCACCCGGGACAACCTGGCTGGTGTGACCAGTCTCAG8390.20245455028063725No Hit
GTGCTGGCTACGCGGGGTCCACGGACCAGCATAGCCCGGGTAGG7790.18797627493279667No Hit
CGGTTCGCCATCCGGACCGCCTTCCTGGTGATACTCGAAGTAGT7530.1817023556153991No Hit
CTGACCACGGTGTTTACCGAACCAACCACCTTTTTTGCGACGGC7350.1773588730110469No Hit
GGTGGACGGGCCTTCGCCCGGGTCGTCAGCCGGACCCTGCTCGA7100.17132625828278003No Hit
TTGTGGCACCGGTGGCAGCAGGGTGGCCATGACACGCTGTTCCA6830.16481103437625177No Hit
ACGACGACCGCCGTCGCCCTGACCACGTGGGCCGGTGGACGGAC6810.16432842519799043No Hit
CGGACGAGTCGGCAGGGTAGCACCGGTTTCGTTCGCGGTCAGTG6750.16288059766320634No Hit
ATACGGATAAGAACTACGGGCAGTGGCTGTAACCCTCTTAACCG6670.16095016095016096No Hit
GTCAGACGGACGAGTTGGCAGGGTTGCGCCGGTTTCGTTTGCGG6670.16095016095016096No Hit
AACCCTCTTAACCGCTGTATCGGCGAGAAGCTCTTCTGAGGGGC6600.15926102882624624No Hit
AGGAGCCCAGGACGGACGCGGAGTGGTCGGCTGGGTCGCAGACT6460.15588276457841674No Hit
ACCGCCATGAGTACCTTTGCAGCCAATACAAGACGGGCGTTTTT5930.1430936213544909No Hit
ACCCAGGGTGATCTGCTGAACACGATCGGAGTAGCCGCACGCTT5890.14212840299796822No Hit
GGCAATATTTAAATATGCTCGTCAGGTGGACGGACCTTCGCCCG5710.13778492039361606No Hit
TGGTGCCCAGGAAGGGCGCGGGGTAGTCGGCTGGGTAGCAGACT5560.1341653515566559No Hit
GGCCTGTTCCTGGTTGGTAATGGACTGCGGAGTTTCGACTTCAA5540.13368274237839456No Hit
ACCCAGCGTGATAGAACGCACGCGGTCAGAGTAACCACATTCCT5480.1322349148436105No Hit
CACAGCCGGCGGACCGGTATCAGACGGAGAGACGGTCGGTGGCG5470.13199361025447984No Hit
GTTGACCCATTCGCCGTTAGCGTTAACACCATAGCCGTCAACGG5070.12234142668925277No Hit
GGCGCGCGGTGGGGTCTGCATAGTCGCCGGTGCCCACTGACGCA5000.12065229456533805No Hit
CCACTGTTCCGGAACCAGTGGACCTTCCATCGGCTGCTGAGGCA4990.12041098997620737No Hit
ACGAACAATCACCTTAATTGCTTTGCCGTTAACTTTAACTACGT4980.1201696853870767No Hit
GTGCGGGTAGATCGGCAGGTTGCCCAGCAGAGTGCCGTCGAAGT4970.11992838079794603No Hit
GTGGGAGTTACGGCATACCGGTGCCGCGTTAGATGGACCCGGGG4860.11727403031750858No Hit
CAGACGGTCGGTTGGATCCGGATACGCGCCGCGCAGCGGTGCTT4780.11534359360446317No Hit
GGTGGACGGACCTTCGCCCGGGTCATCCGCCGGGCCCTGTTCGA4590.11075880641098032No Hit
GATGGTGGATTCGCTACGGGAACGGCGCTGCACAACGTGACGGG4490.10834576051967357No Hit
ACCACCTTTTTTACGGCGGCCGCCATCACCCTGACCACGTGGAC4360.10520880086097477No Hit
CTGGCCGCGGTGTTTACCGAACCAGCCGCCTTTTTTACGACGGC4340.10472619168271342No Hit
GTTCAGACCGTACAGAATACGGCGGTGAACTGGTTTCAGGCCGT4340.10472619168271342No Hit
CATGCTGTCCTGCAGCGGCATCGGTGGTGGCGGGAAGCGGGTAG4210.10158923202401463No Hit
CAGCTCGTCTTCGTCGCCCGCTTCATCTTCGTTGTGCAGCGGGG4190.10110662284575328No Hit
AATCGGCTGGGCTTTACCGGCGTCCATAACAGACAGAGCACATA4170.10062401366749192No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)