FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007958401

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007958401
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences414414
Sequences flagged as poor quality0
Sequence length31
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC31850.7685551163812033No Hit
ATCGAAGTGATTGACGTTGAAACCACTGAAG30960.7470790079485732No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA30840.7441833528790051No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG27140.6549006549006549No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA26910.6493506493506493No Hit
CTGGAACGTCCGGTGTACCCGAAACCGGTTC24100.5815440598049294No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21510.5190461712200843No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA18700.4512395816743643No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG18200.4391743522178305No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT17630.4254199906373819No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG15760.3802960324699455No Hit
GTGGCACAGTCCCAGTACTTCGACCTGCCGC15240.36774819383515034No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA14920.3600264469829687No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC13940.33637859724816244No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG13910.33565468348077043No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC13460.32479597696989No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC13310.32117640813292986No Hit
ACTAGCGAACCGTGCGAAGCGCTGGACCTGT13020.31417857504814023No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC12470.3009068226459531No Hit
CACCCAGGTGTGCCGGTCTCTCCAGCCGTTA12220.2948742079176862No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA12160.2934263803829021No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG11820.28522202435245914No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG11180.26977853064809587No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG11130.2685720077024425No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG10500.2533698185872099No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG10370.2502328589285111No Hit
ATCGTCACCCCACCGTCCGCGCGTCCGCGTA10360.24999155433938045No Hit
ACCCTGTCCCTGTCTCAGCTGTACTCCCACA10330.2492676405719884No Hit
GGTTACCAGGAACCGCCGGCACCACAGGCGC10310.24878503139372704No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG10250.247337203858943No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA9960.2403393707741534No Hit
GGCTACCAGGAACCACCGGCACCACAAGCCC9230.22272413576761405No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC8800.21234803843499495No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG8540.2060741191175974No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT8490.204867596171944No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT8450.2039023778154213No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC8030.1937675850719329No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT7950.1918371483588875No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC7440.179530614313223No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT7340.17711756842191625No Hit
CCGCGTATCATGGCACCGCCGGTAGTTCGTA6940.16746538485668921No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC6720.16215668389581434No Hit
CCGTGGCAGCCGCTGCCGGGTCCGCAGGTGA6690.1614327701284223No Hit
GGTGGTGATAACCATGGCCGTGGCCGTGGCC6370.15371102327624067No Hit
ATGGCAGAACTGCCGCAGAGCCGTATCAACG6360.15346971868711No Hit
CCGCGTATTATGGCGCCGCCGGTTGTACGTA6210.14985014985014986No Hit
GACGCCGCGTCCAGCAGCTCTGCAGGTCAGT5990.14454144888927498No Hit
GATGCGGCCTCCAATTCTGCAAACCGTCAGG5800.13995666169579213No Hit
GTGGTAGAAATGGAGGGTTACCGTTACGTGG5650.136337092858832No Hit
AACGGTGACATGGCTACCGGCTGGGTGAAGG5560.1341653515566559No Hit
ATTGAAGTTATCGACGTTGAAACCACTGAAG5310.128132736828389No Hit
GTGGCCCCGCTGCGTGCGTCCATGGGTCCGG5300.12789143223925833No Hit
ATTGAGCAGAAACAGCTGCTGCAGGGTGATG5260.12692621388273562No Hit
CCGGTAGGCGAAGCAGACTACTTCGAGTATC5240.12644360470447427No Hit
CGCGGCCGTGGTAAAGGTAAGTCTCGTGATA5160.12451316799142885No Hit
ACCAGCGGTAATGTTAGCGTTGGTTATAACC5100.1230653404566448No Hit
CCGGCTGCCTCCCCGCCGCGTACCCCTGCCT5020.12113490374359939No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC5020.12113490374359939No Hit
CCACGTCGCCCGCCGCCGGGCCGTCGTCCTT5020.12113490374359939No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA5010.12089359915446872No Hit
GTAGGTCCAACCCCTCACCAGACCCCGCAGG4960.11968707620881534No Hit
GCCGAAGCTGATTATTTCGAATACCACCAGG4900.11823924867403128No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC4800.11582620278272453No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG4580.11051750182184965No Hit
GCCGGTGATTCCCCAACTCCGGGCCCGTCCA4560.1100348926435883No Hit
CCGCGCATTATGGCCCCTCCGGTTGTACGCA4450.10738054216315085No Hit
GCTCTGGTCTCCGCCATGGAACGCACCGAAC4420.10665662839575883No Hit
ATCGTCACCCCGCCTTCCGCACGCCCGCGTA4380.10569141003923613No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA4330.10448488709358275No Hit
CAGTGCTTCTGGGAAATGCGTGCAGGCCGCG4300.10376097332619072No Hit
CAGAAACAGCTGCTGCAGGGTGACGTGGAAG4250.10255445038053733No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)