FastQCFastQC Report
Wed 31 May 2023
EGAF00007958402

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007958402
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences423251
Sequences flagged as poor quality0
Sequence length44
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AACCGCCGGCGGGCCGGTATCGCTTGGGGATACGGTTGGCGGTG33890.8007069091390215No Hit
CGGCGCACCGTGGTTGATCGGCTGGGTCAGCGGCAGGTCGAAGT27840.6577657229398158No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT26960.6369742776744769No Hit
GCGGACGGACATCTGAGACGGGGAACGCGGTGGGTTCGGGGTCC23660.5590063579294556No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC23300.5505007666845442No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA17840.4214992994700544No Hit
CTGAACAACATCGGTACCCTGAGAAGGCGGTGCGCCCTGAAACA16050.3792076096689671No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA15930.37637241258732995No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA15700.37093828484752545No Hit
AGAACTGAGCCTTCACTGGTTCAGCTCCCTCGGTCGAGTACGCT14960.35345456951076315No Hit
GGCGCCCGCGGTTCAACCAATGGTAACACAAGAACTGAGCCTTC14200.33549832132706126No Hit
ATCGCCCGGAACTACCTGGCTAGTGTGACCGGTCTCGGCAGCGG13440.31754207314335936No Hit
CGCCTGCGGGCACGGACGACCGTGAATGGACAGATCCAGTGCTT13190.31163541255661537No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT12960.30620128481681086No Hit
GACCGGCCATTCCGGGGTGCGCGGACGTGGACGACCGTGAATAG12800.3024210220412946No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT12750.30123968992394584No Hit
CGGCGGCGGTTCCTGATAACCCTGGTACGGGGCTTGCGGAGCCG12620.29816822641883894No Hit
TGGCGGCGGTTCCTGGTAGCCCTGATACGGTGCCTGCGGCGCCG12410.2932066315259739No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG12220.2887175694800485No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA11240.26556345998001185No Hit
CATGGTGTCGCCCGGCACTACCTGGGAAGTGTGGCCGGTCTCAG11140.26320079574531424No Hit
GTGCTGGGAACGTGGGGTCCACGGGCCTGCATAACCCGGGTAGG10720.25327760595958426No Hit
GATTTCCGGGGTTTTGACAGAACGCAGCAGGTCAGCGATGGCCG9970.23555762419935214No Hit
CATGGTGTCACCCGGGACAACCTGGCTGGTGTGACCAGTCTCAG9940.23484882492894288No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG9920.23437629208200336No Hit
CGAGATGTTTCCATCCAGAGGTCTCCGCAGCGGAAAAAATTGGC9890.23366749281159407No Hit
GTCACCCGGAACGACCTGGCTGGTGTGGCCAGTTTCTGCTGCGG9550.22563443441362216No Hit
ACCCTGACCGCGCGGGCCGGTGGACGGGCCTTCGCCCGGGTCAT9520.2249256351432129No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT9350.22090910594422694No Hit
GAAGATGTAATCCCAGGACTCATCCAGGTCAGCCGGATCGATAG9150.21618377747483175No Hit
GCTCGGCTCCTGCTGCATCTGGGTGATTTCGCGGCCAGCACGCA8620.2036616570309344No Hit
GTGCTGGCTACGCGGGGTCCACGGACCAGCATAGCCCGGGTAGG8330.19680993075031128No Hit
CGGTTCGCCATCCGGACCGCCTTCCTGGTGATACTCGAAGTAGT7920.18712300738805107No Hit
CTGACCACGGTGTTTACCGAACCAACCACCTTTTTTGCGACGGC7400.17483715336762345No Hit
GGTGGACGGGCCTTCGCCCGGGTCGTCAGCCGGACCCTGCTCGA7120.16822169351047014No Hit
CGGACGAGTCGGCAGGGTAGCACCGGTTTCGTTCGCGGTCAGTG7050.16656782854618182No Hit
AACCCTCTTAACCGCTGTATCGGCGAGAAGCTCTTCTGAGGGGC6770.15995236868902848No Hit
ATACGGATAAGAACTACGGGCAGTGGCTGTAACCCTCTTAACCG6700.15829850372474016No Hit
ACGACGACCGCCGTCGCCCTGACCACGTGGGCCGGTGGACGGAC6700.15829850372474016No Hit
TTGTGGCACCGGTGGCAGCAGGGTGGCCATGACACGCTGTTCCA6620.15640837233698207No Hit
GTCAGACGGACGAGTTGGCAGGGTTGCGCCGGTTTCGTTTGCGG6560.1549907737961635No Hit
AGGAGCCCAGGACGGACGCGGAGTGGTCGGCTGGGTCGCAGACT6500.15357317525534492No Hit
GGCAATATTTAAATATGCTCGTCAGGTGGACGGACCTTCGCCCG6230.14719398182166138No Hit
ACCCAGGGTGATCTGCTGAACACGATCGGAGTAGCCGCACGCTT6040.14270491977573593No Hit
TGGTGCCCAGGAAGGGCGCGGGGTAGTCGGCTGGGTAGCAGACT5800.13703452561246163No Hit
GGCCTGTTCCTGGTTGGTAATGGACTGCGGAGTTTCGACTTCAA5660.13372679568388499No Hit
ACCCAGCGTGATAGAACGCACGCGGTCAGAGTAACCACATTCCT5440.12852893436755022No Hit
CAGACGGTCGGTTGGATCCGGATACGCGCCGCGCAGCGGTGCTT5250.12403987232162475No Hit
GTGCGGGTAGATCGGCAGGTTGCCCAGCAGAGTGCCGTCGAAGT5250.12403987232162475No Hit
CACAGCCGGCGGACCGGTATCAGACGGAGAGACGGTCGGTGGCG5170.12214974093386667No Hit
GGCGCGCGGTGGGGTCTGCATAGTCGCCGGTGCCCACTGACGCA5120.12096840881651787No Hit
GTTGACCCATTCGCCGTTAGCGTTAACACCATAGCCGTCAACGG5090.12025960954610856No Hit
ACCGCCATGAGTACCTTTGCAGCCAATACAAGACGGGCGTTTTT5050.11931454385222953No Hit
ACGAACAATCACCTTAATTGCTTTGCCGTTAACTTTAACTACGT4920.11624308034712263No Hit
GTGGGAGTTACGGCATACCGGTGCCGCGTTAGATGGACCCGGGG4860.11482548180630406No Hit
CCACTGTTCCGGAACCAGTGGACCTTCCATCGGCTGCTGAGGCA4760.11246281757160645No Hit
ACCACCTTTTTTACGGCGGCCGCCATCACCCTGACCACGTGGAC4600.10868255479609026No Hit
GTTCAGACCGTACAGAATACGGCGGTGAACTGGTTTCAGGCCGT4530.10702868983180193No Hit
CATGGTATCACCTGGAACAACCTGGCTAGTGTGGCCGGTTTCAG4520.10679242340833217No Hit
GATGGTGGATTCGCTACGGGAACGGCGCTGCACAACGTGACGGG4470.10561109129098338No Hit
CTGGCCGCGGTGTTTACCGAACCAGCCGCCTTTTTTACGACGGC4470.10561109129098338No Hit
GGTGGACGGACCTTCGCCCGGGTCATCCGCCGGGCCCTGTTCGA4430.10466602559710432No Hit
CAGCTCGTCTTCGTCGCCCGCTTCATCTTCGTTGTGCAGCGGGG4390.10372095990322527No Hit
CTGGCCACGGTGTTTGCCGAACCAGCCACCTTTTTTACGACGAC4290.10135829566852766No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)