FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007958403

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007958403
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences423251
Sequences flagged as poor quality0
Sequence length31
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGAAGTGATTGACGTTGAAACCACTGAAG33210.7846407923430778No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA31950.7548712229858878No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC31710.7492008288226135No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA28000.6615459857153321No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG27640.6530403944704206No Hit
CTGGAACGTCCGGTGTACCCGAAACCGGTTC23630.5582975586590463No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA18780.443708343276212No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT18470.43638408414864943No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG18180.4295323578680263No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG16030.3787350768220276No Hit
GTGGCACAGTCCCAGTACTTCGACCTGCCGC15460.3652678906842512No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA15120.3572348322862793No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14400.3402236497964565No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC14130.3338444563627729No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG14020.3312455257046056No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC14010.3310092592811358No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC13490.3187234052607082No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC13350.31541567533213155No Hit
ACTAGCGAACCGTGCGAAGCGCTGGACCTGT13300.3142343432147827No Hit
CACCCAGGTGTGCCGGTCTCTCCAGCCGTTA13140.31045408043926653No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA12880.30431115342905274No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG12720.30053089065353655No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG11790.27855811327084873No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG11570.27336025195451397No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG11450.2705250548728768No Hit
GGTTACCAGGAACCGCCGGCACCACAGGCGC11350.2681623906381792No Hit
ACCCTGTCCCTGTCTCAGCTGTACTCCCACA11240.26556345998001185No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG10620.25091494172488665No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA10130.23933788697486835No Hit
GGCTACCAGGAACCACCGGCACCACAAGCCC10100.23862908770445906No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG9770.23083229572995692No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG9670.2284696314952593No Hit
ATCGTCACCCCACCGTCCGCGCGTCCGCGTA9510.22468936871974313No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT9100.21500244535748292No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC9060.21405737966360386No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC8460.19988139425541818No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT8250.1949197993625532No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT7870.18594167527070227No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC7730.1826339453421256No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT7300.17247448913292585No Hit
CCGTGGCAGCCGCTGCCGGGTCCGCAGGTGA6760.15971610226555874No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC6700.15829850372474016No Hit
ATGGCAGAACTGCCGCAGAGCCGTATCAACG6620.15640837233698207No Hit
CCGCGTATCATGGCACCGCCGGTAGTTCGTA6610.1561721059135123No Hit
CCGCGTATTATGGCGCCGCCGGTTGTACGTA6560.1549907737961635No Hit
GACGCCGCGTCCAGCAGCTCTGCAGGTCAGT6200.1464851825512521No Hit
GTGGTAGAAATGGAGGGTTACCGTTACGTGG5850.13821585772981043No Hit
GATGCGGCCTCCAATTCTGCAAACCGTCAGG5690.13443559495429425No Hit
GGTGGTGATAACCATGGCCGTGGCCGTGGCC5530.13065533217877806No Hit
AACGGTGACATGGCTACCGGCTGGGTGAAGG5520.13041906575530832No Hit
ACCAGCGGTAATGTTAGCGTTGGTTATAACC5490.12971026648489903No Hit
CCGGCTGCCTCCCCGCCGCGTACCCCTGCCT5470.1292377336379595No Hit
ATTGAAGTTATCGACGTTGAAACCACTGAAG5460.12900146721448974No Hit
ATTGAGCAGAAACAGCTGCTGCAGGGTGATG5340.12616627013285261No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC5240.12380360589815499No Hit
CGCGGCCGTGGTAAAGGTAAGTCTCGTGATA5210.12309480662774572No Hit
CCGGTAGGCGAAGCAGACTACTTCGAGTATC5080.12002334312263882No Hit
GTAGGTCCAACCCCTCACCAGACCCCGCAGG5050.11931454385222953No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC5040.11907827742875976No Hit
GTGGCCCCGCTGCGTGCGTCCATGGGTCCGG4960.11718814604100168No Hit
GCCGAAGCTGATTATTTCGAATACCACCAGG4840.11435294895936454No Hit
CCACGTCGCCCGCCGCCGGGCCGTCGTCCTT4790.11317161684201572No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA4730.11175401830119716No Hit
CAGAAACAGCTGCTGCAGGGTGACGTGGAAG4720.11151775187772739No Hit
GCTCTGGTCTCCGCCATGGAACGCACCGAAC4690.11080895260731811No Hit
CCTGTAGGTGAAGCGGACTACTTCGAGTATC4580.10821002194915073No Hit
ATCGTCACCCCGCCTTCCGCACGCCCGCGTA4540.1072649562552717No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG4510.1065561569848624No Hit
CCGCGCATTATGGCCCCTCCGGTTGTACGCA4500.10631989056139265No Hit
CAGGGCGCGCTGACCCTGTCCCTGCCGAAAC4460.1053748248675136No Hit
GCCGGTGATTCCCCAACTCCGGGCCCGTCCA4420.10442975917363455No Hit
CTGGCTGAAAACGGTCACAACGTATGGGCAC4400.10395722632669505No Hit
CAGTGCTTCTGGGAAATGCGTGCAGGCCGCG4250.10041322997464862No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)