FastQCFastQC Report
Sat 22 Apr 2023
EGAF00007958406

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007958406
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences402515
Sequences flagged as poor quality0
Sequence length44
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA21190.5264400084468902No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA19590.48668993702098057No Hit
AGAACTGAGCCTTCACTGGTTCAGCTCCCTCGGTCGAGTACGCT16590.41215855309740007No Hit
GGCGCCCGCGGTTCAACCAATGGTAACACAAGAACTGAGCCTTC13880.34483186961976575No Hit
CGAGATGTTTCCATCCAGAGGTCTCCGCAGCGGAAAAAATTGGC9970.24769263257269913No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG9100.22607853123486082No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT8630.21440194775349986No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC8190.20347067811137473No Hit
AACCGCCGGCGGGCCGGTATCGCTTGGGGATACGGTTGGCGGTG8090.20098629864725537No Hit
GTTGCGCGGCGGACGAGTACGTACCGGAACGCGCAGACCGCTCA7770.19303628436207346No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG7560.18781908748742282No Hit
AACAGCGGTAGACGGAGCGGAAGAGGCCAGAGCGCCAGTACCCG7310.18160813882712445No Hit
AACCCTCTTAACCGCTGTATCGGCGAGAAGCTCTTCTGAGGGGC7200.17887532141659315No Hit
ACCCAGCGTGATAGAACGCACGCGGTCAGAGTAACCACATTCCT6880.17092530713141127No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT6680.16595654820317254No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA6520.1619815410605816No Hit
ATACGGATAAGAACTACGGGCAGTGGCTGTAACCCTCTTAACCG6510.16173310311416966No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA6370.15825497186440257No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT5610.1393736879370955No Hit
ACCCAGGGTGATCTGCTGAACACGATCGGAGTAGCCGCACGCTT5540.13763462231221196No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA5500.13664087052656423No Hit
CTGGGAGGTCAGGAACACCAGGTAGCTGCGCTGAGCTTCACGGA5140.12769710445573457No Hit
CGGACGAGTCGGCAGGGTAGCACCGGTTTCGTTCGCGGTCAGTG5130.12744866650932263No Hit
TTCCTGGCCTTCTTCACCCTCATCACCGTCACCACCTTCATCGC5030.12496428704520328No Hit
TGCTGGTTTCGCGGCCGGTTTTGCGGTCGGTTTCGCAGCCGGTT4960.12322522142031973No Hit
CTGACCACGGTGTTTACCGAACCAACCACCTTTTTTGCGACGGC4890.1214861557954362No Hit
GTCAGACGGACGAGTTGGCAGGGTTGCGCCGGTTTCGTTTGCGG4830.1199955281169646No Hit
GTTGACCCATTCGCCGTTAGCGTTAACACCATAGCCGTCAACGG4820.11974709017055266No Hit
GCTAGTTATAGCGGGGCCGAGGTTACTAACCAAAGTGTCGGCAC4650.11552364508154976No Hit
GATACGCGGTACACGAGGTTCGCGCGGGGAACGTGGCACACGCG4470.11105176204613493No Hit
ATCAGCACCACGACCGTTCAGGCTCGGCAGTTTCGCGTTAACGA4380.10881582052842752No Hit
CGGTTCGCCATCCGGACCGCCTTCCTGGTGATACTCGAAGTAGT4070.10111424418965753No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA4040.10036893035042173No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)