FastQCFastQC Report
Wed 31 May 2023
EGAF00007958497

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007958497
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences501499
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCGCCACACCCAGAAAGCACCGAAACGTA51531.027519496549345No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG43290.8632120901537192No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT33010.6582266365436421No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC30420.6065814687566675No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG29490.5880370648794913No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG29300.584248423227165No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG28400.5663022259266719No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA24750.49352042576356087No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG23730.47318140215633525No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA21210.42293204971495457No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG20770.41415835325693573No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA19690.39262291649634395No Hit
GAAGAGGCTCCGGCAGTTGATCCAGTGCAGG19540.38963188361292844No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC18750.37387911042694005No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC17590.3507484561285267No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG17290.34476639036169565No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC14300.28514513488561294No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG14010.2793624713110096No Hit
GTGGTAGAAATGGAGGGTTACCGTTACGTGG13780.27477622088977244No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG12810.25543420824368546No Hit
ATTGAGCAGAAACAGCTGCTGCAGGGTGATG11880.2368898043665092No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC11560.23050893421522278No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG11560.23050893421522278No Hit
AACGTAACCACCAACCAGGACGGCACCGTCT10920.21774719391264985No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA10530.2099705084157695No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC9970.19880398565101823No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT9720.19381893084532573No Hit
GTGGGCGGCTCTGATGCACAGAACACCTCTA9630.1920243111152764No Hit
CAGACCCCGGAACAGAGCACCCCGTCCCGCA9100.18145599492720824No Hit
CAGAAACAGCTGCTGCAGGGTGACGTGGAAG8880.17706914669819881No Hit
ATTGCTGCCGTTGGCTCTCCAGTGAAGTCTA8630.17208409189250629No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT8560.17068827654691235No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG8470.16889365681686305No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG8430.16809604804795225No Hit
GGTCGTCAGGGCTCCGATGTCGAACTGGAAT8400.16749784147126914No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG8180.1631109932422597No Hit
GGTTACCAGGAACCGCCGGCACCACAGGCGC7670.15294148143864691No Hit
ACCGCGTCCGGTGAAGAAGTAGCAGTTCTGA7570.15094745951636993No Hit
GTTGCACGCGTCGCCGACACCCTGCCAACGG7540.15034925293968682No Hit
CAGGGCGCGCTGACCCTGTCCCTGCCGAAAC7460.14875403540186521No Hit
GAAAGCGTCGAACGTGCTATGGGTCGTGTTG7450.1485546332096375No Hit
CAGGACTCTCTGCCGGACACTAAAGCCTCTG7290.1453641981339943No Hit
GCTCTGGTCTCCGCCATGGAACGCACCGAAC7010.13978093675161865No Hit
TCTGCTACCGGTCGTTCCGGCACCGTACTGG6960.13878392579048013No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA6950.13858452359825243No Hit
GGTCGTCAGGGCAGCGACGTAGAACTGGAAT6950.13858452359825243No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA6520.13001022933246129No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCTG6450.12861441398686738No Hit
TCCCGCGTTCCGGCTAAAAAACCGCGTAAAC6340.12642098987236267No Hit
ATGGGTCGCGTAGCAGACACTATCGCTCGTG6290.12542397891122414No Hit
GGCTACCAGGAACCACCGGCACCACAAGCCC6190.12342995698894714No Hit
AAAGTGTACAGCCAGAACGGTCTGGTCCTGC6160.12283175041226403No Hit
ACCGCATCCGGTGAAGAAGTAGCTGTTCTGT6120.12203414164335322No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC6120.12203414164335322No Hit
GGTATGAACACCGCGAACACCGCTGGTACTT6060.12083772848998703No Hit
AGCCCGGCTAAATCTGCGCCGCCGTCTCCGG6000.11964131533662081No Hit
GAAGAGGAAGATGACGACTCCAGCGATACTG5860.116849684645433No Hit
TCTCGCGTGCCGGCGAAGAAACCGCGTAAAC5830.11625147806874989No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT5370.10707897722627563No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT5240.1044867487273155No Hit
TGCCCGGACTTCAAGCTGCGTCTGATGAAGG5190.10348973776617701No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG5100.10169511803612769No Hit
CAGGGTGCCCTGACTCTGAGCCTGCCGAAAC5100.10169511803612769No Hit
GAGAAAACGAAACAGACTCTGGCTTCTGTTT5030.10029930269053378No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)