FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007958527

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007958527
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences512500
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCGCCACACCCAGAAAGCACCGAAACGTA25050.48878048780487804No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12150.2370731707317073No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA11510.22458536585365854No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA11400.2224390243902439No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT11040.2154146341463415No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC10270.20039024390243904No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA10150.19804878048780486No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT8800.17170731707317075No Hit
GTGGCACAGTCCCAGTACTTCGACCTGCCGC6880.1342439024390244No Hit
CTGCCGGTTACCGTCACCATCGCCCCGATGT6870.13404878048780489No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT6680.13034146341463415No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA6680.13034146341463415No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG6270.12234146341463414No Hit
GGTCCGGGCATTAACCCAATCAGCAAAACCG6230.1215609756097561No Hit
ATGATGGACCCGAACTCTACCAGCGAAGACG6140.1198048780487805No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG5420.1057560975609756No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA5270.10282926829268293No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)