FastQCFastQC Report
Sun 21 May 2023
EGAF00007958775

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007958775
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences451014
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC53481.1857725037360258No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA50481.1192557215518808No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT40900.9068454637771777No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG36020.7986448314243018No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA28960.6421086706842803No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG26800.5942165875116959No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT26200.5809132310748669No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT24610.5456593365172699No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG22510.4990975889883684No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA22420.4971020855228441No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC20160.44699277627745476No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC20090.4454407180264914No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA19250.42681601901493077No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG18160.4026482548213581No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA14330.31772849623293287No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC13520.29976896504321376No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT13490.2991037972213723No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG12480.27670981388604343No Hit
CCGGTAGGCGAAGCAGACTACTTCGAGTATC12420.27537947824236053No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC11700.2594154505181657No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT11580.2567547792307999No Hit
CGTAAAGCTAAACCGGCAACTAAGCCGGCAG11320.25098999144150735No Hit
GCCGAAGCTGATTATTTCGAATACCACCAGG10280.2279308402843371No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA9670.21440576124022756No Hit
CCTGTAGGTGAAGCGGACTACTTCGAGTATC9540.2115233673455813No Hit
ACTCCGTCTGACGCAGGCGAAACGGTCGCGG8810.19533761701410599No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8580.19023799704665487No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC8170.18114737014815505No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG7820.17338707889333813No Hit
CCACGTCGCCCGCCGCCGGGCCGTCGTCCTT7140.15830994159826522No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT6630.14700208862696057No Hit
TCCTACACCAACGGCAAACTGACTATCACCC6470.14345452691047283No Hit
GGTACCGGCTACGAAAAACGCGATGACTGGC6270.13902007476486317No Hit
GCTAAAACCGCAGATACCCCGGCGACCTCTA6220.13791146172846075No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG6170.13680284869205833No Hit
GAACGCGCACGTGGTCGTGGCCGTGGCCGTG5960.13214667393916818No Hit
GCTCTGGTCTCCGCCATGGAACGCACCGAAC5960.13214667393916818No Hit
GGTCGTCAGGGCTCCGATGTCGAACTGGAAT5890.1305946156882048No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA5590.12394293746979029No Hit
GGTGGTGATAACCATGGCCGTGGCCGTGGCC5520.12239087921882691No Hit
GTGGGCGGCTCTGATGCACAGAACACCTCTA5330.11817814968049772No Hit
ATGTCTGACGAAGGCCCGGGCACCGGTCCGG5250.11640436882225384No Hit
GTTGAAACCCCGCAGCCGACCGCTGTATCCG4670.1035444575999858No Hit
GGTCGTCAGGGCAGCGACGTAGAACTGGAAT4650.10310101238542484No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC4630.10265756717086388No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG4520.10021861849077857No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)