FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007958777

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007958777
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences455589
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC51631.1332582656736665No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA49491.0862861043616068No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT39570.8685459921113109No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG36380.7985267423050162No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA30000.6584882426924267No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG27430.6020777499017754No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT26660.5851765516726699No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT26060.5720067868188213No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA23600.5180107509180423No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG21000.46094176988469876No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA20760.4556738639431593No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC20350.44667452462636276No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC19720.4328462715298218No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG17660.3876300788649419No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA14860.3261711762136487No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC13390.2939052523217198No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT12900.2831499443577435No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG12690.2785405266588965No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT12060.26471227356235555No Hit
CCGGTAGGCGAAGCAGACTACTTCGAGTATC11640.2554934381646616No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC11640.2554934381646616No Hit
CGTAAAGCTAAACCGGCAACTAAGCCGGCAG11210.2460551066860701No Hit
CCTGTAGGTGAAGCGGACTACTTCGAGTATC9870.2166426318458084No Hit
GCCGAAGCTGATTATTTCGAATACCACCAGG9850.21620363968401346No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA9240.20281437874926744No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8760.1922785668661886No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG8750.19205907078529114No Hit
ACTCCGTCTGACGCAGGCGAAACGGTCGCGG8720.1914005825425987No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC8350.1832792275493921No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT7310.16045163513605465No Hit
CCACGTCGCCCGCCGCCGGGCCGTCGTCCTT7050.1547447370327203No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG6740.14794035852489854No Hit
TCCTACACCAACGGCAAACTGACTATCACCC6710.1472818702822061No Hit
GGTACCGGCTACGAAAAACGCGATGACTGGC6200.13608757015643486No Hit
GCTAAAACCGCAGATACCCCGGCGACCTCTA6070.13323412110476768No Hit
GGTCGTCAGGGCTCCGATGTCGAACTGGAAT5870.12884419948681816No Hit
GCTCTGGTCTCCGCCATGGAACGCACCGAAC5640.12379578962617624No Hit
GAACGCGCACGTGGTCGTGGCCGTGGCCGTG5560.12203982097899642No Hit
GGTGGTGATAACCATGGCCGTGGCCGTGGCC5440.11940586800822672No Hit
GTGGGCGGCTCTGATGCACAGAACACCTCTA5150.11304048166219992No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA4950.1086505600442504No Hit
ATGTCTGACGAAGGCCCGGGCACCGGTCCGG4830.1060166070734807No Hit
GGTCGTCAGGGCAGCGACGTAGAACTGGAAT4770.10469963058809585No Hit
CCGAAACGTCTGAACCTGTACATCGATGGTG4710.10338265410271098No Hit
GTTGAAACCCCGCAGCCGACCGCTGTATCCG4710.10338265410271098No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG4560.10009021288924887No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)