FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007958779

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007958779
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences432449
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC50151.1596743199776158No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA46651.0787399207767854No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG38140.8819537101484799No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT37040.8565171846853616No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG33170.7670268632833005No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA27660.6396129948271356No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG26270.6074704762873773No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT25110.5806465039808162No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT23960.554053772814829No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA21570.49878714021769044No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG20670.47797543756604827No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC19680.4550825646492419No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA19310.4465266424480112No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC18360.42455873409350003No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG16530.38224160536849433No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA13750.31795656828897745No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC12850.2971448656373353No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG12750.29483245423159726No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT12080.27933929781315253No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT11630.2689334464873315No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC11180.25852759516151036No Hit
CCGGTAGGCGAAGCAGACTACTTCGAGTATC11020.25482773691232957No Hit
CGTAAAGCTAAACCGGCAACTAAGCCGGCAG10340.23910333935331102No Hit
GCCGAAGCTGATTATTTCGAATACCACCAGG9510.21991032468568547No Hit
CCTGTAGGTGAAGCGGACTACTTCGAGTATC9350.21621046643650466No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA9100.2104294379221596No Hit
ACTCCGTCTGACGCAGGCGAAACGGTCGCGG8430.1949362815037149No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG7700.17805567824182736No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC7630.17643699025781076No Hit
CCACGTCGCCCGCCGCCGGGCCGTCGTCCTT6530.15100046479469256No Hit
TCCTACACCAACGGCAAACTGACTATCACCC6480.14984425909182353No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT6390.14776308882665934No Hit
GCTAAAACCGCAGATACCCCGGCGACCTCTA6270.1449881951397737No Hit
GCTCTGGTCTCCGCCATGGAACGCACCGAAC6150.14221330145288807No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG6100.14105709575001907No Hit
GGTACCGGCTACGAAAAACGCGATGACTGGC5950.13758847864141205No Hit
GGTCGTCAGGGCTCCGATGTCGAACTGGAAT5650.13065124442419798No Hit
GGTGGTGATAACCATGGCCGTGGCCGTGGCC5600.12949503872132898No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA5440.12579518047214816No Hit
GAACGCGCACGTGGTCGTGGCCGTGGCCGTG5380.12440773362870534No Hit
GTGGGCGGCTCTGATGCACAGAACACCTCTA4770.11030202405370344No Hit
GGTCGTCAGGGCAGCGACGTAGAACTGGAAT4600.1063709246639488No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG4490.103827272117637No Hit
CCGAAACGTCTGAACCTGTACATCGATGGTG4460.1031335486959156No Hit
ATGTCTGACGAAGGCCCGGGCACCGGTCCGG4380.10128361957132519No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)