FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007958792

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007958792
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences450835
Sequences flagged as poor quality0
Sequence length44
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGGTGGGTCTTCGGGGTCGGCCACGGCAGTTTACGCGGTTTTT40790.9047656016059091No Hit
CGGATGAGTTTTCGGAGTCGGCCACGGCAGTTTACGCGGCTTCT39430.8745993545310369No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT26340.5842492264353921No Hit
CGGGTGGGTTTTCGGGGTCGGCCAAGGCAGTTTACGCGGCTTTT24760.5492031452748789No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC23520.5216986258830836No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT23260.5159315492364169No Hit
AGAACTGAGCCTTCACTGGTTCAGCTCCCTCGGTCGAGTACGCT20660.45826078276974946No Hit
GATGGTGGATTCGCTACGGGAACGGCGCTGCACAACGTGACGGG18920.41966573136513363No Hit
GGCGCCCGCGGTTCAACCAATGGTAACACAAGAACTGAGCCTTC17740.39349207581487683No Hit
GATGGTGGATTCGGAACGGCTACGACGCTGAACGACGTGGCGGG17450.3870595672474409No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA16810.37286368627103045No Hit
AACGGTTACGCTGCTCTGTTCAGACGGACCCGGGCGTTCCGGGG15620.34646822008051725No Hit
CACGTGACGGGTTTGGACCGTGTCAGACGGTGCCAGCGGGTTGG15140.3358213093482095No Hit
AACGTGACGGGTCTGAACGGTGTCAGACGGGGCCAGCGGGTTGG14580.32339991349385033No Hit
AACGGTGACGCTAGACTGTTCGGAAGGACCCGGACGTTCTGGAG13780.3056550622733373No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT12440.2759324364789779No Hit
GGTAGAAGCGGCGCGGGAGCTACCAGACAGGCGACCCGGTGCGG12210.2708307917530804No Hit
TTTCAGGGACGGTACGCCGACACGAGAACCGCGACCTTTAACTG12030.26683820022846494No Hit
AGACGCTAGATGAGGAGCGTACGGAGCTGAACACAGATTTAAAT11920.2643982831856444No Hit
GACGCTGCTCTGTTCAGACGGACCCGGACGTTCAGGGGTGGACT11870.2632892299843623No Hit
GGCATCGGTGAAGCCAGTGCCGAATGCGTCACCCAGACCTTTGA11440.2537513724533366No Hit
CGAGATGTTTCCATCCAGAGGTCTCCGCAGCGGAAAAAATTGGC11200.2484279170871827No Hit
CTGAACGACGTGACGGGTCTGTACAGTGTCAGACGGGGCCAGCG10910.24199540851974669No Hit
TACGTGACGGGTCTGCACGGTGTCAGACGGCGCCAGCGGGTTGG10840.24044273403795183No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG10630.23578471059256711No Hit
CGGCGGAACCGGGACAGTAGCCTGCGCGGTGGTCACCGGTGGGG10550.2340102254705158No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT10120.22447236793949008No Hit
GGAGCCCAGGCTGTCGAACGCTGGGTGCAGCAGAGCCAGACCTT10040.22269788281743877No Hit
CTGCACTACGTGACGGGTCTGGACGGTATCAGACGGCGCCAGCG9120.20229130391384875No Hit
CTGGACTACGTGACGCGTCTGAACGGTATCGCTCGGCGCCAGCG9060.20096044007231026No Hit
ACCCTGACCGCGCGGGCCGGTGGACGGGCCTTCGCCCGGGTCAT8840.19608060598666918No Hit
CTGACCACGGTGTTTACCGAACCAACCACCTTTTTTGCGACGGC8670.19230982510231018No Hit
AACCCTCTTAACCGCTGTATCGGCGAGAAGCTCTTCTGAGGGGC8630.19142258254128452No Hit
TGCGTCTTCGAAATCCTCGTCATCGTCATCTTCTTCTTCACGCA8290.18388102077256646No Hit
ATACGGATAAGAACTACGGGCAGTGGCTGTAACCCTCTTAACCG8020.1778921334856433No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG7960.17656126964410482No Hit
GCGAGTCTGATCGGTGATAACATAACGAGTTTCGATCATATCTT7960.17656126964410482No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA7820.17345592068051505No Hit
CACGCTAGACTGCTCGGACGGACCTGGACGTTCTGGGGTAGACT7660.16990695043641244No Hit
ACGAACAATCACCTTAATTGCTTTGCCGTTAACTTTAACTACGT7460.16547073763128417No Hit
TGCCCCATTTAAATCAGCCTGTCACTGGACAACGTGACGGGTCT7250.1608127141858995No Hit
AACGCTAGACTGTTCGCTCGGACCAGGACGTTCTGGAGTGGACT7170.1590382290638482No Hit
ACCACCTTTTTTACGGCGGCCGCCATCACCCTGACCACGTGGAC7030.1559328801002584No Hit
GTTGGTAGCGCCGGTTTCCACCGCGGTCAGCGCCGGAACTTCTT6740.14950037153282245No Hit
GGTGGACGGGCCTTCGCCCGGGTCGTCAGCCGGACCCTGCTCGA6710.14883493961205319No Hit
AGTGGTTTTGTGAACGATACGGACCTTCTGCGGCACGCCACGCA6560.145507780008207No Hit
TGCATCTTCGAAGTCTTCATCATCGTCGTCGTCTTCTTCTTCAC6420.14240243104461722No Hit
CGGACGAGTCGGCAGGGTAGCACCGGTTTCGTTCGCGGTCAGTG6140.13619173311743762No Hit
CGGCGAAGCGACCGTTGAAAAAGGTGGATGCAACTGATTTAAAT6020.1335300054343607No Hit
CGTACTCCTGGCGACAGAGAAAATGGCGGTGACGTTATTTAAAT5900.13086827775128373No Hit
CCCATGACGGATTCCTGTCACCGAAGGATGCGTACTCCTGGCGA5500.1219958521410272No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT5430.12044317765923232No Hit
GTCAGACGGACGAGTTGGCAGGGTTGCGCCGGTTTCGTTTGCGG5430.12044317765923232No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA5200.11534153293333482No Hit
CTGGCCGCGGTGTTTACCGAACCAGCCGCCTTTTTTACGACGGC5190.11511972229307839No Hit
GGCAATATTTAAATATGCTCGTCAGGTGGACGGACCTTCGCCCG4940.10957445628666806No Hit
CTGGCCACGGTGTTTGCCGAACCAGCCACCTTTTTTACGACGAC4910.10890902436589885No Hit
AGAGGACGGACGGCTACCCTGTTTGCCGTTCCAGTAGGCCTGTG4830.10713453924384751No Hit
GTAACCCTGCGCGATTTTAATCTGAGAGACACCGTATTTATCGG4830.10713453924384751No Hit
GTACTCCAGTCGGTGTTTCCGCCCTCTACGCGGCGAAGCGACCG4820.1069127286035911No Hit
GCTCAGCTGAGACGGCTGCATGTTTTTGCCGGCCAGGTGTGCTT4820.1069127286035911No Hit
GGCCGGGTAGGTTGGAGTGGTGGAAGCTGCACGGGAAGAGCCGG4680.10380737964000132No Hit
GGTGGACGGACCTTCGCCCGGGTCATCCGCCGGGCCCTGTTCGA4530.10048022003615513No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)