FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007958793

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007958793
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences450835
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCGCCACACCCAGAAAGCACCGAAACGTA41860.9284993401133453No Hit
GGTCGTCAGGGCAGCGACGTAGAACTGGAAT39660.8797009992569345No Hit
GGTCGTCAGGGCTCCGATGTCGAACTGGAAT39350.8728248694089856No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC29160.6467998269877007No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG27620.612640988388213No Hit
GGTCGTCAGGGCTCCGACGTCGAGCTGGAAA24930.5529739261592379No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC24460.5425488260671865No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT23080.5119389577118014No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19680.436523340024621No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG19330.42875996761564655No Hit
CAGGGCGCGCTGACCCTGTCCCTGCCGAAAC18830.4176694356028259No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT18800.41700400368205665No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA18510.41057149511462065No Hit
TCCCGCGTTCCGGCTAAAAAACCGCGTAAAC17090.37907438419821No Hit
CAGGACTCTCTGCCGGACACTAAAGCCTCTG16690.37020195858795346No Hit
TCTCGCGTGCCGGCGAAGAAACCGCGTAAAC16340.362438586178979No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA14670.32539620925615803No Hit
ACCGCATCCGGTGAAGAAGTTGCGGTACTGA14040.311422138920004No Hit
CAGGGTGCCCTGACTCTGAGCCTGCCGAAAC13690.3036587665110295No Hit
ACCGCGTCCGGTGAAGAAGTAGCAGTTCTGA13470.29877893242538844No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG13120.291015560016414No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG12770.28325218760743953No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA12700.2816995131256446No Hit
ACCGCATCCGGTGAAGAAGTAGCTGTTCTGT12580.2790377854425677No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC11690.25929663845974693No Hit
AGCGGTGACTGTGGCGGCATCCTGCGCTGCC11630.25796577461820847No Hit
GCTCTGACCCTGAGCCTGCCGAAACAGCAGG11620.257743963977952No Hit
TCTGCGTCCGCCTCCGTTCTGTCTCCGACCG11420.2533077511728238No Hit
ATGGAGCCAGTTCAGCAGCGCCCGGTTATGT11370.2521986979715417No Hit
GCGTACGCACTGTCTTCTCAGCAGAAAGCAC11270.24998059156897756No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT10580.23467565739128507No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC10240.227134095622567No Hit
GCGCTGACTCTGTCCCTGCCGAAACAGCAGG10050.22291969345769516No Hit
CGTGACACCACTCACATTTCCCAGAGCGCAC9960.22092339769538746No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG9780.216930806170772No Hit
GCACTGACCCTGAGCCTGCCGAAACAGCAGG9550.2118291614448745No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG9390.2082801912007719No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG9240.2049530315969257No Hit
ACCCAGAACCCAGTGGAAAACTACATCGACT8790.1949715527853871No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA8350.18521188461410493No Hit
GCCGTCCGTTATTTCCGCGGCCGCGGCTCCG8260.1832155888517972No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT7570.16791065467410474No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA7340.1628090099482072No Hit
ATGGAACCGGTCCAGCAGCGTCCGGTTATGT7320.1623653886676944No Hit
ATGGAACCTGTGCAGCAGCGCCCGGTAATGT7320.1623653886676944No Hit
GCCGAAGCTGATTATTTCGAATACCACCAGG7290.16169995674692517No Hit
GTGCGTTATTTCCGTGGCCGCGGCAGCGGTT7130.15815098650282253No Hit
GCAAACCCGCTGCACGAAGAATTTCGTGCCC6610.14661683320948907No Hit
CCGGTAGGCGAAGCAGACTACTTCGAGTATC6370.14129337784333515No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC6140.13619173311743762No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG5800.1286501713487196No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC5530.12266128406179645No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG5300.11755963933589894No Hit
CCTGTAGGTGAAGCGGACTACTTCGAGTATC5240.11622877549436048No Hit
CTGGTGGACTACCAGGATAAGATGATCAAAG5220.11578515421384764No Hit
AGCCCGGCTAAATCTGCGCCGCCGTCTCCGG4980.11046169884769373No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT4940.10957445628666806No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC4840.10735634988410395No Hit
AACTACCCGCGTGATTCTAAAACCATTATCA4640.10292013707897567No Hit
CCACGTCGCCCGCCGCCGGGCCGTCGTCCTT4610.10225470515820644No Hit
GCTGAAGAGACTACCATCGAAAACGGCATCT4580.10158927323743722No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)