FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007958794

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007958794
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences430270
Sequences flagged as poor quality0
Sequence length44
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGGTGGGTCTTCGGGGTCGGCCACGGCAGTTTACGCGGTTTTT39340.9143096195412183No Hit
CGGATGAGTTTTCGGAGTCGGCCACGGCAGTTTACGCGGCTTCT37750.8773560787412555No Hit
CGGGTGGGTTTTCGGGGTCGGCCAAGGCAGTTTACGCGGCTTTT24110.5603458293629581No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT24030.5584865317126456No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC22690.5273432960699096No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT21260.4941083505705719No Hit
AGAACTGAGCCTTCACTGGTTCAGCTCCCTCGGTCGAGTACGCT19520.45366862667627306No Hit
GATGGTGGATTCGCTACGGGAACGGCGCTGCACAACGTGACGGG18100.4206660933832245No Hit
AACGGTTACGCTGCTCTGTTCAGACGGACCCGGGCGTTCCGGGG16510.3837125525832617No Hit
GGCGCCCGCGGTTCAACCAATGGTAACACAAGAACTGAGCCTTC16500.3834801403769726No Hit
GATGGTGGATTCGGAACGGCTACGACGCTGAACGACGTGGCGGG16450.3823180793455272No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA15740.36581681269900296No Hit
CACGTGACGGGTTTGGACCGTGTCAGACGGTGCCAGCGGGTTGG15000.34861830943361144No Hit
GGTAGAAGCGGCGCGGGAGCTACCAGACAGGCGACCCGGTGCGG13230.3074813489204453No Hit
AACGGTGACGCTAGACTGTTCGGAAGGACCCGGACGTTCTGGAG13200.3067841123015781No Hit
AACGTGACGGGTCTGAACGGTGTCAGACGGGGCCAGCGGGTTGG12880.2993469217003277No Hit
TTTCAGGGACGGTACGCCGACACGAGAACCGCGACCTTTAACTG11400.2649499151695447No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT11210.2605340832500523No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG11190.26006925883747417No Hit
AGACGCTAGATGAGGAGCGTACGGAGCTGAACACAGATTTAAAT11140.2589071978060288No Hit
CGGCGGAACCGGGACAGTAGCCTGCGCGGTGGTCACCGGTGGGG11120.2584423733934506No Hit
GACGCTGCTCTGTTCAGACGGACCCGGACGTTCAGGGGTGGACT11040.25658307574313804No Hit
CGAGATGTTTCCATCCAGAGGTCTCCGCAGCGGAAAAAATTGGC10890.2530968926488019No Hit
CTGAACGACGTGACGGGTCTGTACAGTGTCAGACGGGGCCAGCG10490.24380040439723893No Hit
GGAGCCCAGGCTGTCGAACGCTGGGTGCAGCAGAGCCAGACCTT10240.23799009924001208No Hit
GGCATCGGTGAAGCCAGTGCCGAATGCGTCACCCAGACCTTTGA9900.23008808422618357No Hit
TACGTGACGGGTCTGCACGGTGTCAGACGGCGCCAGCGGGTTGG9450.21962953494317522No Hit
ACCCTGACCGCGCGGGCCGGTGGACGGGCCTTCGCCCGGGTCAT9230.21451646640481556No Hit
CTGCACTACGTGACGGGTCTGGACGGTATCAGACGGCGCCAGCG8980.20870616124758873No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT8960.20824133683501056No Hit
AACCCTCTTAACCGCTGTATCGGCGAGAAGCTCTTCTGAGGGGC8460.19662072652055687No Hit
TGCGTCTTCGAAATCCTCGTCATCGTCATCTTCTTCTTCACGCA8130.1889511237130174No Hit
GCGAGTCTGATCGGTGATAACATAACGAGTTTCGATCATATCTT8040.18685941385641575No Hit
CTGGACTACGTGACGCGTCTGAACGGTATCGCTCGGCGCCAGCG7960.18500011620610315No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG7830.1819787575243452No Hit
TGCCCCATTTAAATCAGCCTGTCACTGGACAACGTGACGGGTCT7800.18128152090547794No Hit
CTGACCACGGTGTTTACCGAACCAACCACCTTTTTTGCGACGGC7760.18035187208032166No Hit
ACGAACAATCACCTTAATTGCTTTGCCGTTAACTTTAACTACGT7430.17268226927278219No Hit
CACGCTAGACTGCTCGGACGGACCTGGACGTTCTGGGGTAGACT7370.17128779603504776No Hit
AACGCTAGACTGTTCGCTCGGACCAGGACGTTCTGGAGTGGACT6940.16129407116461755No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA6920.1608292467520394No Hit
ATACGGATAAGAACTACGGGCAGTGGCTGTAACCCTCTTAACCG6910.16059683454575036No Hit
ACCACCTTTTTTACGGCGGCCGCCATCACCCTGACCACGTGGAC6430.14944104864387478No Hit
AGTGGTTTTGTGAACGATACGGACCTTCTGCGGCACGCCACGCA6380.14827898761242939No Hit
GTTGGTAGCGCCGGTTTCCACCGCGGTCAGCGCCGGAACTTCTT6280.14595486554953865No Hit
GGTGGACGGGCCTTCGCCCGGGTCGTCAGCCGGACCCTGCTCGA6100.14177144583633533No Hit
CGGACGAGTCGGCAGGGTAGCACCGGTTTCGTTCGCGGTCAGTG5760.1338694308225068No Hit
CGGCGAAGCGACCGTTGAAAAAGGTGGATGCAACTGATTTAAAT5750.1336370186162177No Hit
TGCATCTTCGAAGTCTTCATCATCGTCGTCGTCTTCTTCTTCAC5620.13061565993445975No Hit
CGTACTCCTGGCGACAGAGAAAATGGCGGTGACGTTATTTAAAT5480.1273618890464127No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT5230.12155158388918587No Hit
CCCATGACGGATTCCTGTCACCGAAGGATGCGTACTCCTGGCGA5200.12085434727031863No Hit
GTCAGACGGACGAGTTGGCAGGGTTGCGCCGGTTTCGTTTGCGG5170.12015711065145142No Hit
GGCAATATTTAAATATGCTCGTCAGGTGGACGGACCTTCGCCCG5010.11643851535082624No Hit
CTGGCCGCGGTGTTTACCGAACCAGCCGCCTTTTTTACGACGGC4950.11504404211309179No Hit
CTGGCCACGGTGTTTGCCGAACCAGCCACCTTTTTTACGACGAC4870.1131847444627792No Hit
GCTCAGCTGAGACGGCTGCATGTTTTTGCCGGCCAGGTGTGCTT4790.11132544681246659No Hit
AGAGGACGGACGGCTACCCTGTTTGCCGTTCCAGTAGGCCTGTG4660.10830408813070863No Hit
GGCCGGGTAGGTTGGAGTGGTGGAAGCTGCACGGGAAGAGCCGG4660.10830408813070863No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA4610.10714202709926324No Hit
ATCGGCGTCTGCGGTGACACCCATATCTTCCGCAGTCATCTGAA4570.10621237827410696No Hit
GTAACCCTGCGCGATTTTAATCTGAGAGACACCGTATTTATCGG4380.10179654635461455No Hit
ACCAGTAGCAACTACCGCCAGATCGCGGTTCGGGTACGGTGCAA4310.10016966091059103No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)