FastQCFastQC Report
Wed 31 May 2023
EGAF00007958810

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007958810
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences546163
Sequences flagged as poor quality0
Sequence length44
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA29870.5469063264995981No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT29600.5419627473849382No Hit
GGCGCCCGCGGTTCAACCAATGGTAACACAAGAACTGAGCCTTC27120.4965550577391731No Hit
AGAACTGAGCCTTCACTGGTTCAGCTCCCTCGGTCGAGTACGCT26990.49417481594322576No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA25510.46707667857397883No Hit
GGTAGAAGCGGCGCGGGAGCTACCAGACAGGCGACCCGGTGCGG22150.4055565829248777No Hit
CGAGATGTTTCCATCCAGAGGTCTCCGCAGCGGAAAAAATTGGC19900.3643600903027118No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG18530.33927600368388194No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG15990.29276974090152574No Hit
TTTCAGGGACGGTACGCCGACACGAGAACCGCGACCTTTAACTG13390.24516490498257848No Hit
AACCCTCTTAACCGCTGTATCGGCGAGAAGCTCTTCTGAGGGGC13200.24168609005004002No Hit
AGACGCTAGATGAGGAGCGTACGGAGCTGAACACAGATTTAAAT12480.2285032124109469No Hit
ATACGGATAAGAACTACGGGCAGTGGCTGTAACCCTCTTAACCG12130.22209486911416557No Hit
GAAGATGTAATCCCAGGACTCATCCAGGTCAGCCGGATCGATAG11620.21275699745314128No Hit
TGCGTCTTCGAAATCCTCGTCATCGTCATCTTCTTCTTCACGCA11250.20598246311082954No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT10770.19719387801810082No Hit
CGGACGAGTCGGCAGGGTAGCACCGGTTTCGTTCGCGGTCAGTG10190.18657433769772028No Hit
GTCAGACGGACGAGTTGGCAGGGTTGCGCCGGTTTCGTTTGCGG10070.1843771914245381No Hit
GGTTGCCAGCGGACCAATGATAGTCGGCGGGGTTTTCGGACGGC9870.18071528096923448No Hit
TTTCTGCAGGATGTTCTGTACCGCGTCGGACGGGGTTTTGGCAC8610.15764524510082153No Hit
AGAGGACGGACGGCTACCCTGTTTGCCGTTCCAGTAGGCCTGTG8360.15306785703169198No Hit
TGACCTATTTAAATACGAGCCTCAGTCAGACGGACGAGTCGGCA8300.15196928389510092No Hit
ACCAATGATGGTCGGCGGGGTTTTCGGACGAGAGGTTTCCGGAC8060.14757499134873656No Hit
GTTGCGCGGCGGACGAGTACGTACCGGAACGCGCAGACCGCTCA7970.1459271316438499No Hit
GCTAGTTATAGCGGGGCCGAGGTTACTAACCAAAGTGTCGGCAC7610.1393356928243034No Hit
GGCCGGGTAGGTTGGAGTGGTGGAAGCTGCACGGGAAGAGCCGG7420.13585687789176493No Hit
GTGGGAGTTACGGCATACCGGTGCCGCGTTAGATGGACCCGGGG7090.1298147256405139No Hit
ATCGCCCGGAACTACCTGGCTAGTGTGACCGGTCTCGGCAGCGG7080.12963163011774873No Hit
CGGCGGCGGTTCCTGATAACCCTGGTACGGGGCTTGCGGAGCCG6990.1279837704128621No Hit
TGCATCTTCGAAGTCTTCATCATCGTCGTCGTCTTCTTCTTCAC6710.12285709577543699No Hit
CGTACTCCTGGCGACAGAGAAAATGGCGGTGACGTTATTTAAAT6610.12102614054778518No Hit
TGCTGGTTTCGCGGCCGGTTTTGCGGTCGGTTTCGCAGCCGGTT6460.11827970770630747No Hit
CCCATGACGGATTCCTGTCACCGAAGGATGCGTACTCCTGGCGA6450.11809661218354227No Hit
GCTCAGCTGAGACGGCTGCATGTTTTTGCCGGCCAGGTGTGCTT6420.11754732561524674No Hit
CATGGTGTCGCCCGGCACTACCTGGGAAGTGTGGCCGGTCTCAG6340.11608256143312529No Hit
GTACTCCAGTCGGTGTTTCCGCCCTCTACGCGGCGAAGCGACCG6100.11168826888676091No Hit
CGGCGAAGCGACCGTTGAAAAAGGTGGATGCAACTGATTTAAAT6080.11132207784123056No Hit
GAAGCCACCTTTGGAGCGGGTTTTAGAATCACGCTTACGGCGCA5930.10857564499975282No Hit
GCGAATGTATGCCAGCCACGCTTCGTCTTCAGCCAGGATATCGC5840.10692778529486618No Hit
GTCGCCCGGCAGCGGTGCAGTCGGAGCCCACGGGGAGTTACCGT5840.10692778529486618No Hit
CTGTGCGCCCTACGCGGAGGTGGCCTATTCATCGGTGCAGGATT5820.10656159424933581No Hit
CGGTTTATCTTTCTCGACGTCTTCCGCCAGCGGGAATGCCGGGC5720.104730639021684No Hit
GGACTGGTCCGGCAGGGTATCCGGGAAAGCCGCACGTGCCGCAC5480.10033634647531964No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)