FastQCFastQC Report
Sun 21 May 2023
EGAF00007958812

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007958812
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences523929
Sequences flagged as poor quality0
Sequence length44
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA28600.5458754907630614No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT28510.5441577007571636No Hit
AGAACTGAGCCTTCACTGGTTCAGCTCCCTCGGTCGAGTACGCT26570.5071297828522567No Hit
GGCGCCCGCGGTTCAACCAATGGTAACACAAGAACTGAGCCTTC26380.5035033372842503No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA24640.47029273050356063No Hit
GGTAGAAGCGGCGCGGGAGCTACCAGACAGGCGACCCGGTGCGG20940.3996724747055422No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG18780.35844551456399626No Hit
CGAGATGTTTCCATCCAGAGGTCTCCGCAGCGGAAAAAATTGGC17860.3408858833925971No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG16180.3088204699491725No Hit
TTTCAGGGACGGTACGCCGACACGAGAACCGCGACCTTTAACTG12960.24736176084927536No Hit
AACCCTCTTAACCGCTGTATCGGCGAGAAGCTCTTCTGAGGGGC12190.2326651130210391No Hit
ATACGGATAAGAACTACGGGCAGTGGCTGTAACCCTCTTAACCG11780.22483962521639383No Hit
AGACGCTAGATGAGGAGCGTACGGAGCTGAACACAGATTTAAAT11710.2235035663229178No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT11010.21014297738815757No Hit
GAAGATGTAATCCCAGGACTCATCCAGGTCAGCCGGATCGATAG10700.20422614514562087No Hit
GTCAGACGGACGAGTTGGCAGGGTTGCGCCGGTTTCGTTTGCGG10330.19716411956581903No Hit
TGCGTCTTCGAAATCCTCGTCATCGTCATCTTCTTCTTCACGCA10190.19449200177886697No Hit
CGGACGAGTCGGCAGGGTAGCACCGGTTTCGTTCGCGGTCAGTG9430.17998621950684157No Hit
GGTTGCCAGCGGACCAATGATAGTCGGCGGGGTTTTCGGACGGC9100.17368765615188317No Hit
TTTCTGCAGGATGTTCTGTACCGCGTCGGACGGGGTTTTGGCAC8310.1586092772112252No Hit
TGCATCTTCGAAGTCTTCATCATCGTCGTCGTCTTCTTCTTCAC8260.15765494943017086No Hit
ACCAATGATGGTCGGCGGGGTTTTCGGACGAGAGGTTTCCGGAC7940.15154725163142335No Hit
AGAGGACGGACGGCTACCCTGTTTGCCGTTCCAGTAGGCCTGTG7790.14868426828826042No Hit
GCTAGTTATAGCGGGGCCGAGGTTACTAACCAAAGTGTCGGCAC7540.14391262938298893No Hit
TGACCTATTTAAATACGAGCCTCAGTCAGACGGACGAGTCGGCA7510.14334003271435633No Hit
GTTGCGCGGCGGACGAGTACGTACCGGAACGCGCAGACCGCTCA7400.14124051159603687No Hit
GGCCGGGTAGGTTGGAGTGGTGGAAGCTGCACGGGAAGAGCCGG7200.13742320047181966No Hit
GTGGGAGTTACGGCATACCGGTGCCGCGTTAGATGGACCCGGGG7150.13646887269076535No Hit
CGTACTCCTGGCGACAGAGAAAATGGCGGTGACGTTATTTAAAT6870.13112463711686126No Hit
CGGCGGCGGTTCCTGATAACCCTGGTACGGGGCTTGCGGAGCCG6750.1288342504423309No Hit
CCCATGACGGATTCCTGTCACCGAAGGATGCGTACTCCTGGCGA6650.12692559488022231No Hit
ATCGCCCGGAACTACCTGGCTAGTGTGACCGGTCTCGGCAGCGG6530.12463520820569199No Hit
GCTCAGCTGAGACGGCTGCATGTTTTTGCCGGCCAGGTGTGCTT6500.1240626115370594No Hit
TGCTGGTTTCGCGGCCGGTTTTGCGGTCGGTTTCGCAGCCGGTT6080.11604625817620326No Hit
CGGCGAAGCGACCGTTGAAAAAGGTGGATGCAACTGATTTAAAT5980.11413760261409465No Hit
CGGTTTATCTTTCTCGACGTCTTCCGCCAGCGGGAATGCCGGGC5730.10936596370882315No Hit
GGACTGGTCCGGCAGGGTATCCGGGAAAGCCGCACGTGCCGCAC5720.10917509815261228No Hit
CTGTGCGCCCTACGCGGAGGTGGCCTATTCATCGGTGCAGGATT5640.1076481737029254No Hit
GTCGCCCGGCAGCGGTGCAGTCGGAGCCCACGGGGAGTTACCGT5590.10669384592187109No Hit
GTCACCCGGAACGACCTGGCTGGTGTGGCCAGTTTCTGCTGCGG5460.10421259369112991No Hit
GAAGCCACCTTTGGAGCGGGTTTTAGAATCACGCTTACGGCGCA5430.10363999702249732No Hit
CATGGTGTCGCCCGGCACTACCTGGGAAGTGTGGCCGGTCTCAG5390.10287653479765387No Hit
GTACTCCAGTCGGTGTTTCCGCCCTCTACGCGGCGAAGCGACCG5350.10211307257281044No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)