FastQCFastQC Report
Sun 21 May 2023
EGAF00007958813

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007958813
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences523929
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT59281.131451017217982No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA57161.0909875193012795No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG41960.8008718738607713No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT28000.5344235573904098No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA25830.493005731692653No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA23830.4548326204504809No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG21760.41532345031483275No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC19810.3781046668537149No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG19130.36512580903137637No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG18120.3458483878540795No Hit
ACCGCGTCCGGTGAAGAAGTAGCAGTTCTGA15590.29755940213273174No Hit
ACCGCATCCGGTGAAGAAGTTGCGGTACTGA13340.2546146519852881No Hit
ACCGCATCCGGTGAAGAAGTAGCTGTTCTGT13290.2536603242042338No Hit
TCTGCGTCCGCCTCCGTTCTGTCTCCGACCG12480.23820021415115405No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC11710.2235035663229178No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA11550.22044971742354405No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA11120.212242498506477No Hit
GCCGTCCGTTATTTCCGCGGCCGCGGCTCCG10780.20575306959530773No Hit
GATGGCGAACACCTGGAAGGTGGCGACGGCA10250.19563719511613212No Hit
GTGCGTTATTTCCGTGGCCGCGGCAGCGGTT9870.18838430398011946No Hit
CTGCCGCAGTCTCCAGGCCCAGCGTTCCCGC9470.18074968173168504No Hit
ATTGCTGCCGTTGGCTCTCCAGTGAAGTCTA8600.16414437834134016No Hit
CGCGGCCGTGGTAAAGGTAAGTCTCGTGATA7900.1507837894065799No Hit
AGCCCGGCTAAATCTGCGCCGCCGTCTCCGG7880.15040205829415818No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC7770.14830253717583872No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT7600.14505782272025408No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC7590.14486695716404321No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG7010.13379675490381332No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG6940.13246069601033728No Hit
CGTAAAGCTAAACCGGCAACTAAGCCGGCAG6030.11509193039514896No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG6010.11471019928272724No Hit
GGCGAGTTCCGCCTGCACAACCCGATCAAAG5840.11146548482714261No Hit
CACACCCCTCCGATGCCGTCTATCGGTCTGG5740.109556829265034No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG5490.1047851903597625No Hit
TTCGAACAGCCGACCCTGACCTTCGGCGCGG5420.10344913146628645No Hit
TGCCCGGACTTCAAGCTGCGTCTGATGAAGG5340.10192220701659958No Hit
GCCGGTGATTCCCCAACTCCGGGCCCGTCCA5310.101349610347967No Hit
GGCGCACCGTCCGGCGGCCCGAGCGACGTTA5260.10039528256691269No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)