FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007958850

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007958850
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences401839
Sequences flagged as poor quality0
Sequence length44
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AACCGCCGGCGGGCCGGTATCGCTTGGGGATACGGTTGGCGGTG32180.8008182381501049No Hit
CGGCGCACCGTGGTTGATCGGCTGGGTCAGCGGCAGGTCGAAGT26050.648269580603177No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT24650.6134297566936011No Hit
GCGGACGGACATCTGAGACGGGGAACGCGGTGGGTTCGGGGTCC23350.5810784916347094No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC22940.5708754003469051No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA17060.424548139926687No Hit
CTGAACAACATCGGTACCCTGAGAAGGCGGTGCGCCCTGAAACA15170.37751437764875984No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA15150.37701666587862304No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA14520.36133874511931396No Hit
AGAACTGAGCCTTCACTGGTTCAGCTCCCTCGGTCGAGTACGCT14480.36034332157904037No Hit
ATCGCCCGGAACTACCTGGCTAGTGTGACCGGTCTCGGCAGCGG12940.32201951527850703No Hit
GGCGCCCGCGGTTCAACCAATGGTAACACAAGAACTGAGCCTTC12630.31430498284138675No Hit
GACCGGCCATTCCGGGGTGCGCGGACGTGGACGACCGTGAATAG12140.3021110444730352No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT12060.300120197392488No Hit
CGCCTGCGGGCACGGACGACCGTGAATGGACAGATCCAGTGCTT11960.297631638541804No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT11930.2968850708865989No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG11810.2938988002657781No Hit
CGGCGGCGGTTCCTGATAACCCTGGTACGGGGCTTGCGGAGCCG11630.28941939433454694No Hit
TGGCGGCGGTTCCTGGTAGCCCTGATACGGTGCCTGCGGCGCCG11120.2767277441960586No Hit
CATGGTGTCGCCCGGCACTACCTGGGAAGTGTGGCCGGTCTCAG10380.2583124087009972No Hit
GTGCTGGGAACGTGGGGTCCACGGGCCTGCATAACCCGGGTAGG10330.25706812927565514No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA10130.2520910115742872No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG9870.24562075856250887No Hit
GAAGATGTAATCCCAGGACTCATCCAGGTCAGCCGGATCGATAG9640.2398970732059357No Hit
CGAGATGTTTCCATCCAGAGGTCTCCGCAGCGGAAAAAATTGGC9590.23865279378059373No Hit
GATTTCCGGGGTTTTGACAGAACGCAGCAGGTCAGCGATGGCCG9240.22994283780319977No Hit
ACCCTGACCGCGCGGGCCGGTGGACGGGCCTTCGCCCGGGTCAT9110.22670771129731063No Hit
GTCACCCGGAACGACCTGGCTGGTGTGGCCAGTTTCTGCTGCGG8950.22272601713621623No Hit
GCTCGGCTCCTGCTGCATCTGGGTGATTTCGCGGCCAGCACGCA8780.21849546709005346No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT8740.21750004354977986No Hit
CATGGTGTCACCCGGGACAACCTGGCTGGTGTGACCAGTCTCAG8390.20879008757238596No Hit
CGGTTCGCCATCCGGACCGCCTTCCTGGTGATACTCGAAGTAGT8080.20107555513526562No Hit
GTGCTGGCTACGCGGGGTCCACGGACCAGCATAGCCCGGGTAGG7750.19286331092800849No Hit
CTGACCACGGTGTTTACCGAACCAACCACCTTTTTTGCGACGGC6970.17345255189267342No Hit
GGTGGACGGGCCTTCGCCCGGGTCGTCAGCCGGACCCTGCTCGA6900.17171056069719465No Hit
CGGACGAGTCGGCAGGGTAGCACCGGTTTCGTTCGCGGTCAGTG6650.1654891635704847No Hit
ATACGGATAAGAACTACGGGCAGTGGCTGTAACCCTCTTAACCG6290.15653035170802235No Hit
TTGTGGCACCGGTGGCAGCAGGGTGGCCATGACACGCTGTTCCA6280.15628149582295395No Hit
AGGAGCCCAGGACGGACGCGGAGTGGTCGGCTGGGTCGCAGACT6250.15553492816774878No Hit
AACCCTCTTAACCGCTGTATCGGCGAGAAGCTCTTCTGAGGGGC6210.15453950462747518No Hit
ACGACGACCGCCGTCGCCCTGACCACGTGGGCCGGTGGACGGAC6160.1532952252021332No Hit
GGCAATATTTAAATATGCTCGTCAGGTGGACGGACCTTCGCCCG6000.1493135310410388No Hit
GTCAGACGGACGAGTTGGCAGGGTTGCGCCGGTTTCGTTTGCGG5990.1490646751559704No Hit
CACAGCCGGCGGACCGGTATCAGACGGAGAGACGGTCGGTGGCG5630.1401058632935081No Hit
TGGTGCCCAGGAAGGGCGCGGGGTAGTCGGCTGGGTAGCAGACT5620.1398570074084397No Hit
ACCCAGGGTGATCTGCTGAACACGATCGGAGTAGCCGCACGCTT5550.1381150162129609No Hit
ACCCAGCGTGATAGAACGCACGCGGTCAGAGTAACCACATTCCT5220.12990277200570377No Hit
GGCGCGCGGTGGGGTCTGCATAGTCGCCGGTGCCCACTGACGCA5210.12965391612063537No Hit
ACCGCCATGAGTACCTTTGCAGCCAATACAAGACGGGCGTTTTT5190.1291562043504986No Hit
GGCCTGTTCCTGGTTGGTAATGGACTGCGGAGTTTCGACTTCAA5180.1289073484654302No Hit
CAGACGGTCGGTTGGATCCGGATACGCGCCGCGCAGCGGTGCTT5030.1251745101894042No Hit
GTGCGGGTAGATCGGCAGGTTGCCCAGCAGAGTGCCGTCGAAGT4920.12243709545365185No Hit
CCACTGTTCCGGAACCAGTGGACCTTCCATCGGCTGCTGAGGCA4760.11845540129255747No Hit
GTGGGAGTTACGGCATACCGGTGCCGCGTTAGATGGACCCGGGG4720.11745997775228388No Hit
GTTGACCCATTCGCCGTTAGCGTTAACACCATAGCCGTCAACGG4690.11671341009707868No Hit
ACGAACAATCACCTTAATTGCTTTGCCGTTAACTTTAACTACGT4560.1134782835911895No Hit
AATCGGCTGGGCTTTACCGGCGTCCATAACAGACAGAGCACATA4400.10949658943009515No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT4320.10750574234954795No Hit
ACCACCTTTTTTACGGCGGCCGCCATCACCCTGACCACGTGGAC4310.10725688646447955No Hit
GATGGTGGATTCGCTACGGGAACGGCGCTGCACAACGTGACGGG4270.10626146292420596No Hit
CTGGCCGCGGTGTTTACCGAACCAGCCGCCTTTTTTACGACGGC4270.10626146292420596No Hit
GGTGGACGGACCTTCGCCCGGGTCATCCGCCGGGCCCTGTTCGA4240.10551489526900078No Hit
CAGCTCGTCTTCGTCGCCCGCTTCATCTTCGTTGTGCAGCGGGG4240.10551489526900078No Hit
CTGGCACAGAGAATCACGGTTGGAGCGTTTGGTCGCTTCGTCCA4150.10327519230338518No Hit
TGGAGCCCAAGACGGACGCGGGGTGGTCGGCTGGGTTGCAGACT4130.10277748053324838No Hit
GTTCAGACCGTACAGAATACGGCGGTGAACTGGTTTCAGGCCGT4090.1017820569929748No Hit
GCTTGGTTCCTGTTGCATCTGAGTGATTTCACGACCAGCGCGCA4080.1015332011079064No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)