FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007958856

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007958856
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences389170
Sequences flagged as poor quality0
Sequence length44
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA20490.5265051262944215No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA18750.48179458848318213No Hit
AGAACTGAGCCTTCACTGGTTCAGCTCCCTCGGTCGAGTACGCT16190.41601356733561173No Hit
GGCGCCCGCGGTTCAACCAATGGTAACACAAGAACTGAGCCTTC13210.3394403474060179No Hit
CGAGATGTTTCCATCCAGAGGTCTCCGCAGCGGAAAAAATTGGC9270.23819924454608526No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG8520.21892746100675795No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC8210.21096179047716937No Hit
AACCGCCGGCGGGCCGGTATCGCTTGGGGATACGGTTGGCGGTG7940.20402394840301155No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT7850.20171133437829228No Hit
AACCCTCTTAACCGCTGTATCGGCGAGAAGCTCTTCTGAGGGGC7030.1806408510419611No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG7010.1801269368142457No Hit
GTTGCGCGGCGGACGAGTACGTACCGGAACGCGCAGACCGCTCA6900.17730040856181103No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA6880.17678649433409563No Hit
AACAGCGGTAGACGGAGCGGAAGAGGCCAGAGCGCCAGTACCCG6760.17370300896780327No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA6230.1600842819333453No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT6230.1600842819333453No Hit
ATACGGATAAGAACTACGGGCAGTGGCTGTAACCCTCTTAACCG6160.15828558213634145No Hit
ACCCAGCGTGATAGAACGCACGCGGTCAGAGTAACCACATTCCT6070.15597296811162217No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA5720.14697946912660276No Hit
ACCCAGGGTGATCTGCTGAACACGATCGGAGTAGCCGCACGCTT5410.13901379859701415No Hit
CGGACGAGTCGGCAGGGTAGCACCGGTTTCGTTCGCGGTCAGTG5090.13079117095356785No Hit
TGCTGGTTTCGCGGCCGGTTTTGCGGTCGGTTTCGCAGCCGGTT5010.12873551404270628No Hit
CTGGGAGGTCAGGAACACCAGGTAGCTGCGCTGAGCTTCACGGA4920.126422900017987No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT4900.1259089857902716No Hit
ATCAGCACCACGACCGTTCAGGCTCGGCAGTTTCGCGTTAACGA4800.12333941465169462No Hit
TTCCTGGCCTTCTTCACCCTCATCACCGTCACCACCTTCATCGC4710.12102680062697536No Hit
GCTAGTTATAGCGGGGCCGAGGTTACTAACCAAAGTGTCGGCAC4460.11460287278053294No Hit
GATACGCGGTACACGAGGTTCGCGCGGGGAACGTGGCACACGCG4380.11254721586967135No Hit
GTTGACCCATTCGCCGTTAGCGTTAACACCATAGCCGTCAACGG4300.11049155895880977No Hit
CTGACCACGGTGTTTACCGAACCAACCACCTTTTTTGCGACGGC4280.10997764473109437No Hit
GTCAGACGGACGAGTTGGCAGGGTTGCGCCGGTTTCGTTTGCGG4260.10946373050337897No Hit
CTGTGCGCCCTACGCGGAGGTGGCCTATTCATCGGTGCAGGATT4030.10355371688465194No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA4010.10303980265693655No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT3930.10098414574607499No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)