FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007958926

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007958926
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences461185
Sequences flagged as poor quality0
Sequence length44
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAACTGAGCCTTCACTGGTTCAGCTCCCTCGGTCGAGTACGCT50081.0858982837689863No Hit
GGCGCCCGCGGTTCAACCAATGGTAACACAAGAACTGAGCCTTC49981.083729956525039No Hit
CGAGATGTTTCCATCCAGAGGTCTCCGCAGCGGAAAAAATTGGC42270.9165519260166745No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC34970.7582640372084954No Hit
ATACGGATAAGAACTACGGGCAGTGGCTGTAACCCTCTTAACCG28630.620792089942214No Hit
AACCCTCTTAACCGCTGTATCGGCGAGAAGCTCTTCTGAGGGGC23890.5180133785790951No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA23500.5095569023276992No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT22600.4900419571321704No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA21340.4627210338584299No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT20510.4447239177336644No Hit
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA18290.3965870529180264No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA17260.37425328230536553No Hit
GTACATGTGCGGACGGTGCGGATGGTTATCGAAACCGTGCGGGT13210.2864360289254854No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT12730.2760280581545367No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA12220.2649695892104036No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT11480.24892396760519098No Hit
GGCAATATTTAAATATGCTCGTCAGGTGGACGGACCTTCGCCCG11090.24046749135379514No Hit
GCGACGTTTCGTGCTCTGTTTGGTAGAGCTTTTGGTGGTTGCTG9720.21076140811171223No Hit
TTACGCGTTGACAATACCGTAGGTCGTCAGCGAGATGTTTCCAT9500.20599108817502737No Hit
CTGACCACGGTGTTTACCGAACCAACCACCTTTTTTGCGACGGC8230.17845333217689213No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA8190.17758600127931307No Hit
TGGTGCCTGAGACTCGTTCAGTTTCTTAGCTTCGCCCAGGACGT8040.1743335104133916No Hit
GCCTGCGGTCTGGCCGTTCGCGGCTTCATAAGACCAGGACCAGG7700.16696119778396956No Hit
CGGTTCGCCATCCGGACCGCCTTCCTGGTGATACTCGAAGTAGT7590.16457603781562713No Hit
ACCCTGACCGCGCGGGCCGGTGGACGGGCCTTCGCCCGGGTCAT7290.15807105608378416No Hit
GCTCAGCTGAGACGGCTGCATGTTTTTGCCGGCCAGGTGTGCTT6980.15134924162754643No Hit
GGTGGACGGACCTTCGCCCGGGTCATCCGCCGGGCCCTGTTCGA6740.14614525624207206No Hit
AGAGGACGGACGGCTACCCTGTTTGCCGTTCCAGTAGGCCTGTG6690.14506109262009823No Hit
CGGGCCTTCGCCCGGGTCATCAGCCGGGCCCTGTTCGATCGCAC6560.14224226720296626No Hit
ACCACCTTTTTTACGGCGGCCGCCATCACCCTGACCACGTGGAC6340.13747194726628142No Hit
GGTGGACGGGCCTTCGCCCGGGTCGTCAGCCGGACCCTGCTCGA6190.13421945640035993No Hit
ACCTGCGGAGGTAACGTTGTTGTTGGTTTTCGGAGCGGTGGTGT6170.13378579095157042No Hit
TGTGACATTTAAATTGGAGTATCAGGTAGACGGGCCTTCGCCCG5800.1257629801489641No Hit
CGCCTGCGGGCACGGACGACCGTGAATGGACAGATCCAGTGCTT5780.12532931470017455No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT5720.12402831835380596No Hit
GAAGATGTAATCCCAGGACTCATCCAGGTCAGCCGGATCGATAG5680.1231609874562269No Hit
ACCGTGCTGGGAGAACAGGCGACCGGCGCTTTTCGGGTTTGCCT5540.12012532931470017No Hit
AGACGGCGGCGGGGTAGGTTTGACCGGAACTGGTTTCGGCACTG5370.11643917299998915No Hit
AGCGCCGGTCGGAGGCGGGCAAACGTAGAAGTTTGCGTCGGTGT5170.11210251851209384No Hit
CTGGCCGCGGTGTTTACCGAACCAGCCGCCTTTTTTACGACGGC5090.11036785671693572No Hit
GCTAGTTATAGCGGGGCCGAGGTTACTAACCAAAGTGTCGGCAC4910.10646486767782995No Hit
CACCTGACCATTGGCGTGAGTGGTAACGTTGTAGGCGTTAGTTT4770.10342920953630322No Hit
CTGGCCACGGTGTTTGCCGAACCAGCCACCTTTTTTACGACGAC4720.10234504591432939No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)