FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007958927

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007958927
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences461185
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCGCCACACCCAGAAAGCACCGAAACGTA109642.3773539902642105No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA57501.2467881652699024No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA55121.1951819768639482No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC48931.0609625204635884No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT40780.8842438500818544No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA25780.5589947634897059No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT23260.504352916942225No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT22420.48613896809306456No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG20100.43583377603347895No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC13590.29467567245248655No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG13390.2903390179645912No Hit
GAATGCGACAACTATCGCCAGGAATACGATA13260.28752019254745925No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC12900.2797142144692477No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA11670.25304378936869154No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT10060.21813372074113427No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC9680.20989407721413314No Hit
ATCACTCTGTCTCAAAACGGCTACAACACTG8970.1944989537821048No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG8930.19363162288452573No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA8320.18040482669644503No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG8250.17888699762568167No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG8030.17411667768899683No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG8030.17411667768899683No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC8010.17368301224020727No Hit
GCGGAAGTGAATGTTGACCAGGCTCACCTGG7800.16912952502791723No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7680.16652753233518003No Hit
CCACGTCGCCCGCCGCCGGGCCGTCGTCCTT7570.1641423723668376No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC7380.16002255060333706No Hit
CCGGATCCGGCTGTTGCCCCGACCAGCGCGG6960.1509155761787569No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT6950.15069874345436213No Hit
GCCGAAGCTGATTATTTCGAATACCACCAGG6840.1483135834860197No Hit
GACCCAGCTATCGTTCAGCCGAAAGCAGCAA6550.14202543447857152No Hit
CCGGTAGGCGAAGCAGACTACTTCGAGTATC6520.1413749363053872No Hit
CTGGGCTGGAAAGCTGGTATCCTGACCGGTT6290.1363877836443076No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA5850.12684714377093792No Hit
CACCCAGGTGTGCCGGTCTCTCCAGCCGTTA5770.1251124819757798No Hit
AACGTAACCACCAACCAGGACGGCACCGTCT5600.12142632566106877No Hit
CCGAAGAAAAACCGTAAACCGAAGCCACCGA5590.12120949293667399No Hit
GCAGAAGCGCAGTCCACTCCGGAGCGTCCTG5480.11882433296833159No Hit
CCTGTAGGTGAAGCGGACTACTTCGAGTATC5130.11123518761451479No Hit
CCGGACCCAGCGGTGGCACCGACTTCTGCGG4900.10624803495343517No Hit
CAGGTTAGCTATGGTGCTCGCCCGACCCAGA4790.10386287498509274No Hit
GTACTGCCGACCCCGATGCAGCTGGCACTGC4650.10082721684356603No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)