FastQCFastQC Report
Wed 31 May 2023
EGAF00007958928

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007958928
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences443052
Sequences flagged as poor quality0
Sequence length44
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAACTGAGCCTTCACTGGTTCAGCTCCCTCGGTCGAGTACGCT48561.0960338741276419No Hit
GGCGCCCGCGGTTCAACCAATGGTAACACAAGAACTGAGCCTTC47521.072560331518648No Hit
CGAGATGTTTCCATCCAGAGGTCTCCGCAGCGGAAAAAATTGGC40460.9132110903460542No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC33130.7477677563807409No Hit
ATACGGATAAGAACTACGGGCAGTGGCTGTAACCCTCTTAACCG27300.6161804934860919No Hit
AACCCTCTTAACCGCTGTATCGGCGAGAAGCTCTTCTGAGGGGC23310.5261233444381247No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT22420.5060354089361971No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA21880.49384722335075787No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA19120.4315520525807354No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT18220.4112384099383368No Hit
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA17080.38550779592463186No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA15830.3572944033657449No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA12270.27694266135803475No Hit
GTACATGTGCGGACGGTGCGGATGGTTATCGAAACCGTGCGGGT12250.27649124707709255No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT12100.2731056399700261No Hit
GGCAATATTTAAATATGCTCGTCAGGTGGACGGACCTTCGCCCG10780.24331229742784144No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT10530.23766961891606406No Hit
GCGACGTTTCGTGCTCTGTTTGGTAGAGCTTTTGGTGGTTGCTG10300.23247835468522882No Hit
TTACGCGTTGACAATACCGTAGGTCGTCAGCGAGATGTTTCCAT9180.20719915495246605No Hit
ACCCTGACCGCGCGGGCCGGTGGACGGGCCTTCGCCCGGGTCAT8050.18169424807923223No Hit
TGGTGCCTGAGACTCGTTCAGTTTCTTAGCTTCGCCCAGGACGT7720.17424591244368606No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA7540.17018318391520634No Hit
CTGACCACGGTGTTTACCGAACCAACCACCTTTTTTGCGACGGC7440.16792611251049538No Hit
GGTGGACGGGCCTTCGCCCGGGTCGTCAGCCGGACCCTGCTCGA7380.1665718696676688No Hit
GCCTGCGGTCTGGCCGTTCGCGGCTTCATAAGACCAGGACCAGG7230.16318626256060237No Hit
CGGTTCGCCATCCGGACCGCCTTCCTGGTGATACTCGAAGTAGT7190.16228343399871797No Hit
GCTCAGCTGAGACGGCTGCATGTTTTTGCCGGCCAGGTGTGCTT6390.1442268627610303No Hit
ACCTGCGGAGGTAACGTTGTTGTTGGTTTTCGGAGCGGTGGTGT6190.1397127199516084No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT6110.13790706282783963No Hit
ACCACCTTTTTTACGGCGGCCGCCATCACCCTGACCACGTGGAC6110.13790706282783963No Hit
AGAGGACGGACGGCTACCCTGTTTGCCGTTCCAGTAGGCCTGTG6040.13632711284454196No Hit
GGTGGACGGACCTTCGCCCGGGTCATCCGCCGGGCCCTGTTCGA5920.13361862715888878No Hit
CGGGCCTTCGCCCGGGTCATCAGCCGGGCCCTGTTCGATCGCAC5860.13226438431606222No Hit
GAAGATGTAATCCCAGGACTCATCCAGGTCAGCCGGATCGATAG5650.1275245343661692No Hit
TGTGACATTTAAATTGGAGTATCAGGTAGACGGGCCTTCGCCCG5630.127073120085227No Hit
CGCCTGCGGGCACGGACGACCGTGAATGGACAGATCCAGTGCTT5510.12436463439957388No Hit
ACCGTGCTGGGAGAACAGGCGACCGGCGCTTTTCGGGTTTGCCT5330.12030190587109413No Hit
AGACGGCGGCGGGGTAGGTTTGACCGGAACTGGTTTCGGCACTG5110.11533634878073003No Hit
CACCTGACCATTGGCGTGAGTGGTAACGTTGTAGGCGTTAGTTT4970.11217644881413469No Hit
CTGGCCGCGGTGTTTACCGAACCAGCCGCCTTTTTTACGACGGC4910.11082220597130811No Hit
GCTAGTTATAGCGGGGCCGAGGTTACTAACCAAAGTGTCGGCAC4590.10359957747623302No Hit
AGCGCCGGTCGGAGGCGGGCAAACGTAGAAGTTTGCGTCGGTGT4530.10224533463340647No Hit
TTTGGTCACACGCGGACCGTAAGTCGCGGTGCCGTCGGCGTGGG4510.10179392035246428No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)